Analytical Data
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Gene name
coaE
- Application
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Alternative Names
Dephosphocoenzyme A kinase
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Species
Escherichia coli
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Source
E. coli
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Tag
N- His-SUMO & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A6I9
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Expression Region
1-206aa
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Molecular Weight
42.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CoaE is a key protein involved in the biosynthesis of coenzyme A (CoA), which plays a critical role in various metabolic pathways, including fatty acid oxidation and the citric acid cycle. Research into CoaE has gained attention due to its potential implications in metabolic disorders and its importance in cell metabolism regulation. The enzyme catalyzes the conversion of phosphopantetheine to CoA, making it an essential component in the CoA synthesis pathway. Understanding the structural and functional characteristics of CoaE can provide insights into its catalytic mechanism, regulatory functions, and interactions with other biomolecules. Additionally, since CoA is vital for the metabolism of lipids and carbohydrates, studies on CoaE may reveal new therapeutic targets for diseases linked to energy metabolism, such as obesity, diabetes, and certain types of cancer. The generation of recombinant CoaE proteins through genetic engineering techniques facilitates detailed biochemical studies, enabling researchers to investigate the enzyme's kinetics, substrate specificity, and potential inhibitors. Overall, the exploration of CoaE and its role in CoA biosynthesis is a promising field of study that could contribute to our understanding of metabolic regulation and disease prevention.
