Analytical Data
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Gene name
entC3
- Application
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Alternative Names
SEC3
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Species
Staphylococcus aureus
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A0L5
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Expression Region
28-266aa
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Molecular Weight
43.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EntC3 is a recombinant protein that has garnered attention due to its role in iron acquisition and metabolism in pathogenic bacteria. Research into EntC3 is driven by the increasing need to understand iron-siderophore complexes, as many bacterial pathogens rely on such systems to thrive in iron-limited environments, such as within a host. This protein is particularly relevant in the context of bacterial pathogenesis, as it assists in the transportation of iron, which is crucial for various cellular processes. With rising antibiotic resistance, elucidating the mechanisms of iron uptake can lead to the development of novel therapeutic strategies aimed at disrupting iron acquisition systems in bacteria. Studies on EntC3 not only provide insights into its structural and functional characteristics but also offer potential avenues for the design of new antimicrobial agents. Through advancements in recombinant DNA technology, researchers have been able to produce EntC3 in vitro, allowing for detailed biochemical characterizations and assessments of its interaction with iron and siderophores. Furthermore, understanding the nuances of EntC3's function could pave the way for innovative approaches to target bacterial infections that exploit iron acquisition pathways, making this protein a significant focus in the field of microbial pathogenesis and antibiotic development.
