Analytical Data
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Gene name
pbpC
- Application
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Alternative Names
PBP 3 Alternative name(s): PSPB20 Penicillin-binding protein C
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Species
Bacillus subtilis
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P42971
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Expression Region
21-240aa
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Molecular Weight
29.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PbpC recombinant protein, derived from the gene encoding penicillin-binding protein C, has garnered significant attention in the field of microbiology and antibiotic resistance research. PbpC plays a critical role in the synthesis of bacterial cell walls, making it an essential target for antibiotic development. Understanding the structure and function of PbpC is crucial, as alterations in its activity can lead to bacterial resistance against beta-lactam antibiotics, which include penicillins and cephalosporins. The emergence of resistant strains poses a major challenge in clinical settings, prompting the need for new therapeutic strategies. By producing PbpC as a recombinant protein, researchers can study its biochemical properties, enzymatic activity, and interactions with various antibiotics in detail. This recombinant form allows for high-yield production and facilitates structural analyses, such as X-ray crystallography or NMR spectroscopy, leading to insights into the molecular mechanisms of resistance. Ultimately, research on PbpC recombinant protein not only enhances our understanding of bacterial cell wall biosynthesis but also informs the design of novel inhibitors, contributing to the fight against antibiotic-resistant infections.
