Analytical Data
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Gene name
EIF1B
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简介
EIF1B Protein likely intricately participates in translation, playing a crucial role in facilitating accurate and efficient protein synthesis within cellular machinery. Its involvement suggests a key function in orchestrating various steps required for proper decoding of mRNA and subsequent assembly of polypeptide chains. EIF1B Protein, Human (His) is the recombinant human-derived EIF1B protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
rHuEukaryotic translation initiation factor 1b/EIF1B, His; Eukaryotic Translation Initiation Factor 1b; eIF1b; Protein Translation Factor SUI1 Homolog GC20; EIF1B
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60739
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Expression Region
M1-F113
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Protein Length
Full Length
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Molecular Weight
16 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF1B, or eukaryotic translation initiation factor 1B, is a crucial protein involved in the initiation of mRNA translation. It plays a key role in the assembly of the pre-initiation complex by facilitating the recruitment of the initiator methionyl-tRNA to the ribosome. Research on EIF1B has gained attention due to its involvement in various cellular processes and its implication in several diseases, including cancer. Abnormal regulation or mutations in the EIF1B gene can disrupt normal protein synthesis, leading to altered cellular function and potentially contributing to oncogenesis. Moreover, EIF1B has been identified as a target in therapeutic strategies aimed at modulating protein synthesis in cancer cells. As a result, recombination technologies for producing EIF1B have been explored to better understand its structure-function relationships and regulatory mechanisms. The recombinant protein can be utilized for functional studies, helping to elucidate its role in translation initiation and interactions with other translation factors. Additionally, studying EIF1B in a recombinant form allows researchers to investigate its potential as a biomarker and therapeutic target in diseases associated with disrupted translation, making it a significant focus in molecular biology and cancer research.
