Analytical Data
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Gene name
PTPRC/CD45RABC
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简介
Receptor-type tyrosine-protein phosphatase C (PTPRC) is a receptor type PTP and an essential positive regulator of T- and B-cell antigen receptor signaling. PTPRC functions through direct interaction with antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. PTPRC also suppresses JAK kinases as a regulator of cytokine receptor signaling. PTPRC/CD45RABC Protein, Human (HEK293, His) is the recombinant human-derived PTPRC/CD45RABC protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48
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Species
Human
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Source
HEK293
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Tag
C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P08575-3
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Expression Region
Q26-K577
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Protein Length
Extracellular Domain
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Molecular Weight
118-150 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PTPRC, also known as CD45, is a crucial receptor-type protein tyrosine phosphatase predominantly expressed on the surface of immune cells, including T and B lymphocytes. It plays a significant role in regulating various cellular processes such as cell activation, proliferation, and differentiation by modulating signal transduction pathways. The CD45 molecule exists in multiple isoforms, including CD45RA, CD45RB, and CD45RO, which are generated through alternative splicing of a single gene. These isoforms are important for the functional status of immune cells, impacting their responses to antigens and overall immune system function. Research into recombinant PTPRC/CD45RABC proteins focuses on understanding the structure-function relationship of these isoforms, their roles in immune responses, and their potential implications in autoimmune diseases, immunotherapy, and vaccine development. By producing and characterizing these recombinant proteins, scientists aim to elucidate the mechanistic pathways involved in T cell activation and signal regulation, ultimately contributing to advancements in targeted therapies for various immunological disorders. The generation of high-purity recombinant CD45 proteins allows for detailed biochemical studies and potential applications in the design of novel immunomodulatory agents, offering new avenues for enhancing immune responses in clinical settings.
