Analytical Data
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Gene name
SLC16A3
- Application
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Alternative Names
SLC16A3; Monocarboxylate transporter 4; Solute carrier family 16 member 3; MCT4; MCT3
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Species
Human
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Source
E. coli Cell-free
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Tag
N-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15427
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Expression Region
M1-V465
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AA Sequence
MGGAVVDEGPTGVKAPDGGWGWAVLFGCFVITGFSYAFPKAVSVFFKELIQEFGIGYSDTAWISSILLAMLYGTGPLCSVCVNRFGCRPVMLVGGLFASLGMVAASFCRSIIQVYLTTGVITGLGLALNFQPSLIMLNRYFSKRRPMANGLAAAGSPVFLCALSPLGQLLQDRYGWRGGFLILGGLLLNCCVCAALMRPLVVTAQPGSGPPRPSRRLLDLSVFRDRGFVLYAVAASVMVLGLFVPPVFVVSYAKDLGVPDTKAAFLLTILGFIDIFARPAAGFVAGLGKVRPYSVYLFSFSMFFNGLADLAGSTAGDYGGLVVFCIFFGISYGMVGALQFEVLMAIVGTHKFSSAIGLVLLMEAVAVLVGPPSGGKLLDATHVYMYVFILAGAEVLTSSLILLLGNFFCIRKKPKEPQPEVAAAEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV
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Protein Length
Full Length
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Molecular Weight
Monomer: 45-50 kDa;Dimer: 120 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SLC16A3, also known as the monocarboxylate transporter 4 (MCT4), is a member of the SLC16 family, which facilitates the transport of lactate and other monocarboxylates across cellular membranes. This protein plays a crucial role in cellular metabolism, particularly in regulating lactate levels during anaerobic glycolysis, which is especially important in highly glycolytic tissues such as muscles and tumors. Elevated expression of SLC16A3 has been implicated in the development of various cancers, as it not only aids in the efflux of lactate, thereby preventing acidosis, but also promotes the Warburg effect, a metabolic shift seen in cancer cells favoring fermentation over oxidative phosphorylation. Understanding the structure and function of SLC16A3 can provide insights into its involvement in metabolic regulation and its potential as a therapeutic target. Research on recombinant SLC16A3 proteins has enabled detailed investigations into the transport mechanisms, substrate specificity, and regulatory pathways that govern its activity. These studies contribute to a better understanding of metabolic disorders and the tumor microenvironment, paving the way for the development of novel strategies aimed at modulating SLC16A3 function in cancer and other diseases.
