Analytical Data
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Gene name
MAP3K12
- Application
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Alternative Names
DLK; MUK; ZPK; ZPKP1; MEKK12; Zipper Protein Kinase; Dual Leucine Zipper Kinase; Leucine-zipper protein kinase; MAPK-upstream kinase; Mixed lineage kinase
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Species
Rat
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q63796
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Expression Region
Ile158~Ile399
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Molecular Weight
28kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MAP3K12, also known as Mitogen-Activated Protein Kinase Kinase Kinase 12, is an important member of the MAPK signaling pathway, which plays a crucial role in various cellular processes, including proliferation, differentiation, and apoptosis. Disruption of this signaling can lead to several diseases, including cancer and inflammatory disorders. Research on MAP3K12 has garnered attention due to its involvement in key signaling cascades, particularly in response to stress and immune challenges. Additionally, MAP3K12 has been shown to interact with various substrates and downstream effectors, highlighting its potential as a therapeutic target. The generation of recombinant MAP3K12 protein is essential for elucidating its biochemical properties and functional roles in detail. This enables scientists to study its enzymatic activity, substrate specificity, and interaction with other proteins. Furthermore, understanding the structure-function relationships of MAP3K12 can reveal insights into how aberrations in this kinase contribute to disease pathology, paving the way for the development of targeted therapies. Overall, the study of recombinant MAP3K12 protein is pivotal in advancing our knowledge of MAPK signaling mechanisms and its implications in health and disease.
