Analytical Data
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基因名
NAMPT
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简介
NAMPT protein has dual functions of cytokine and adipokine. Due to limited ATP activation, its secreted form lacks enzymatic activity. It catalyzes the condensation of nicotinamide and 5-phosphoribosyl-1-pyrophosphate to produce nicotinamide mononucleotide, which is critical for NAD biosynthesis. NAMPT Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived NAMPT protein, expressed by P. pastoris , with N-10*His labeled tag.
- Application
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别名
rHuNicotinamide phosphoribosyltransferase/NAMPT, His; Pre-B cell-enhancing factor; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Visfatin; NAMPT; PBEF; PBEF1
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种属
Mouse
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表达系统
P. pastoris
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标签
N-10*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q99KQ4
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表达区间
M1-H491
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氨基酸序列
MNAAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKVRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKEVYREHFQDDVFNERGWNYILEKYDGHLPIEVKAVPEGSVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGIALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTEAPLIIRPDSGNPLDTVLKVLDILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKKWSIENVSFGSGGALLQKLTRDLLNCSFKCSYVVTNGLGVNVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGHDLLHTVFKNGKVTKSYSFDEVRKNAQLNIEQDVAPH
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蛋白长度
Full Length
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分子量
Apparently 62 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NAMPT (Nicotinamide adenine dinucleotide (NAD) biosynthesis pathway protein 1) is a crucial enzyme involved in the NAD+ salvage pathway, playing a significant role in cellular metabolism, energy production, and regulating various physiological processes. Its activity is essential for maintaining cellular NAD+ levels, which are vital for numerous enzymatic reactions, including those required for DNA repair, cell signaling, and energy metabolism. Dysfunction of NAMPT has been implicated in various pathological conditions, including metabolic disorders, cancer, and neurodegenerative diseases, making it an important target for therapeutic intervention. Researchers are increasingly focusing on the recombinant production of NAMPT to study its functional properties, structural biology, and interactions with other molecules. The recombinant forms are valuable for in vitro assays, enabling the elucidation of its role in NAD+ biosynthesis and potential as a drug target. By dissecting the mechanistic insights into NAMPT's function, scientists aim to develop novel therapeutic strategies that could manipulate NAD+ levels, thereby influencing disease progression and treatment outcomes. The exploration of NAMPT not only enhances our understanding of cellular homeostasis but also opens avenues for novel pharmacological agents, underscoring its significance in both basic and applied biomedical research.
