Analytical Data
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Gene name
NAMPT
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简介
NAMPT protein has dual functions of cytokine and adipokine. Due to limited ATP activation, its secreted form lacks enzymatic activity. It catalyzes the condensation of nicotinamide and 5-phosphoribosyl-1-pyrophosphate to produce nicotinamide mononucleotide, which is critical for NAD biosynthesis. NAMPT Protein, Mouse is the recombinant mouse-derived NAMPT protein, expressed by E. coli , with tag free.
- Application
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Biological Activity
Measured by its ability to induce VEGF secretion by PC-3 human prostate cancer cells. The ED50 for this effect is 1.337 μg/mL. Measured by its ability to induce VEGF secretion by PC-3 human prostate cancer cells. The ED50 for this effect is 1.337 μg/mL, corresponding to a specific activity is 7.48×102 U/mg.
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Alternative Names
rHuNicotinamide phosphoribosyltransferase/NAMPT, His; Pre-B cell-enhancing factor; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Visfatin; NAMPT; PBEF; PBEF1
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Species
Mouse
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q99KQ4
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Expression Region
M1-H491
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AA Sequence
MNAAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKVRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKEVYREHFQDDVFNERGWNYILEKYDGHLPIEVKAVPEGSVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGIALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTEAPLIIRPDSGNPLDTVLKVLDILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKKWSIENVSFGSGGALLQKLTRDLLNCSFKCSYVVTNGLGVNVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGHDLLHTVFKNGKVTKSYSFDEVRKNAQLNIEQDVAPH
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Protein Length
Full Length
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Molecular Weight
56 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NAMPT (Nicotinamide adenine dinucleotide (NAD) biosynthesis pathway protein 1) is a crucial enzyme involved in the NAD+ salvage pathway, playing a significant role in cellular metabolism, energy production, and regulating various physiological processes. Its activity is essential for maintaining cellular NAD+ levels, which are vital for numerous enzymatic reactions, including those required for DNA repair, cell signaling, and energy metabolism. Dysfunction of NAMPT has been implicated in various pathological conditions, including metabolic disorders, cancer, and neurodegenerative diseases, making it an important target for therapeutic intervention. Researchers are increasingly focusing on the recombinant production of NAMPT to study its functional properties, structural biology, and interactions with other molecules. The recombinant forms are valuable for in vitro assays, enabling the elucidation of its role in NAD+ biosynthesis and potential as a drug target. By dissecting the mechanistic insights into NAMPT's function, scientists aim to develop novel therapeutic strategies that could manipulate NAD+ levels, thereby influencing disease progression and treatment outcomes. The exploration of NAMPT not only enhances our understanding of cellular homeostasis but also opens avenues for novel pharmacological agents, underscoring its significance in both basic and applied biomedical research.
