Analytical Data
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Gene name
PRKAa2
- Application
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Alternative Names
AMPK; AMPK2; PRKAA; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-2; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P54646
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Expression Region
Asp252~Ala493
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Molecular Weight
37kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PRKAa2, a protein kinase typically associated with the AMP-activated protein kinase (AMPK) pathway, has garnered significant attention in recent years due to its crucial role in cellular energy homeostasis and metabolism. AMPK acts as a cellular energy sensor, responding to changes in cellular energy levels by activating catabolic processes that generate ATP while inhibiting anabolic processes that consume ATP. Understanding the function of PRKAa2 and its regulatory mechanisms is vital for deciphering its involvement in metabolic disorders, including obesity, type 2 diabetes, and cardiovascular diseases. Research has demonstrated that PRKAa2 can influence various cellular functions, such as glucose uptake, lipid metabolism, and mitochondrial biogenesis, making it a potential therapeutic target for a range of metabolic diseases. Recent studies also highlight its role in the response to oxidative stress and inflammation, suggesting that PRKAa2 may have implications beyond metabolic regulation. The advent of recombinant protein technology enables the production of PRKAa2 in a controlled environment, facilitating detailed biochemical studies and the development of specific inhibitors or activators. Investigating the structure-function relationship of PRKAa2 through recombinant protein expression provides insights into its enzymatic activity and interaction with other cellular pathways. This knowledge could pave the way for novel strategies in drug development aimed at modulating PRKAa2 activity for therapeutic purposes. Overall, ongoing research on PRKAa2 holds the promise of advancing our understanding of metabolic regulation and developing innovative approaches to manage related diseases.
