Analytical Data
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Gene name
Doublecortin/DCX
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简介
Doublecortin/DCX Protein, a microtubule-associated protein, is vital for early stages of neuronal dispersion and cortex lamination during cerebral cortex development. It may compete with the neuronal protein kinase DCLK1 in binding to a target protein, contributing to crucial signaling pathways involved in neuronal interaction and migration. DCX interacts with tubulin and USP9X. Doublecortin/DCX Protein, Human (His) is the recombinant human-derived Doublecortin/DCX protein, expressed by E. coli , with N-GST labeled tag.
- Application
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Alternative Names
Neuronal migration protein doublecortin; Doublin; Lis-X; DCX
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
O43602-2
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Expression Region
A45-V150
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Protein Length
Partial
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Molecular Weight
13 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Doublecortin (DCX) is a microtubule-associated protein that plays a crucial role in neuronal development, particularly in the migration of neuroblasts during cortical development. Mutations in the DCX gene can lead to various forms of lissencephaly, a condition characterized by abnormal cortical development and intellectual disabilities. Our understanding of DCX's structure and function is pivotal for unraveling the mechanisms underlying these developmental disorders. Recombinant DCX protein has become essential for studying its biochemical properties and interactions with other cellular components. By producing DCX in a laboratory setting, researchers can investigate its role in microtubule dynamics and neuronal morphology, assess the impact of disease-associated mutations, and explore potential therapeutic targets for neural regeneration. Furthermore, the characterization of recombinant DCX helps in designing experiments to understand how this protein influences neurogenesis and brain plasticity. Recent advances in protein expression and purification techniques have made it feasible to obtain high-quality recombinant DCX, thus facilitating detailed investigations into its function, interactions, and pathway regulations. Understanding DCX better not only sheds light on fundamental neurodevelopmental processes but also has implications for developing strategies to address neurodevelopmental disorders associated with its dysfunction. This ongoing research underscores the significance of DCX as a critical player in brain development and highlights the potential for therapeutic applications in regenerative medicine and neuroprotection.
