Analytical Data
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基因名
DNASEII
- Application
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别名
DNase-II; DNase2; DNASE2A; DNASE2B; DRN2; DNL; DNL2; Deoxyribonuclease II Alpha; Acid DNase; Lysosomal DNase II
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O00115
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表达区间
Cys19~Ile360
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分子量
42kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNase II is a crucial endonuclease that plays a significant role in DNA degradation and disposal within cellular processes, particularly in lysosomal degradation. Unlike other DNases, DNase II functions optimally in acidic environments, making it essential for the degradation of extracellular DNA, which can arise from cellular debris, apoptotic cells, and pathogen infection. The study of recombinant DNase II proteins has garnered attention due to their potential applications in various fields, including biotechnology, medicine, and genetic research. For instance, understanding the enzyme’s structure and function can lead to innovative therapeutic strategies in treating genetic disorders or enhancing the efficacy of gene therapy. Additionally, recombinant DNase II can serve as a tool for studying cellular processes linked to DNA metabolism, inflammation, and immune responses. Recent advances in molecular biology techniques enable the production and purification of high-quality DNase II recombinant proteins, allowing for detailed biochemical characterization and functional assays. Overall, the research surrounding DNase II recombinant proteins continues to expand, providing insights into their biological significance and potential biotechnological applications.
