Analytical Data
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基因名
EP3
- Application
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别名
PTGER3; EP3-I; EP3-II; EP3-III; EP3-IV; EP3e; PGE2 receptor EP3 subtype; Prostanoid EP3 receptor
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P43115
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表达区间
Arg350~Arg390
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分子量
8kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of EP3 recombinant protein has gained significant attention due to its critical role in the regulation of various physiological processes through the activation of the prostaglandin E3 (PGE3) receptor. Prostaglandins are lipid compounds that have diverse functions, including mediating inflammation, modulating immune responses, and influencing pain pathways. The EP3 receptor, a member of the prostaglandin receptor family, is known for its involvement in several pathophysiological conditions, such as cardiovascular diseases, neurodegenerative disorders, and cancer. Researchers have focused on producing EP3 recombinant protein to better understand its structure-function relationship and to investigate its signaling mechanisms. By utilizing advanced molecular cloning and expression systems, scientists aim to obtain insoluble or soluble forms of the protein, which can then be used in various assays, including binding studies and functional assays, to elucidate its biological roles. Additionally, this research holds potential therapeutic implications, as targeting the EP3 receptor could lead to novel strategies for treating diseases linked to dysregulated prostaglandin signaling. Overall, the investigation of EP3 recombinant protein is pivotal in providing insights into prostaglandin biology and developing targeted therapeutics.
