Analytical Data
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Gene name
PSAP/Prosaposin
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简介
PSAP/Prosaposin protein is a myelin trophic factor and neurotrophic factor that acts through GPR37 and GPR37L1 receptors.This triggers ligand-mediated internalization, initiating ERK phosphorylation signaling.PSAP/Prosaposin Protein, Mouse (HEK293, His) is the recombinant mouse-derived PSAP/Prosaposin protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
Proactivator polypeptide; Saposin-A; PSAP; GLBA; SAP1
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Species
Mouse
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Source
HEK293
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Tag
C-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q61207
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Expression Region
S17-N557
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AA Sequence
SPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN
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Protein Length
Full Length of Mature Protein
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Molecular Weight
61-80 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Prosaposin (PSAP) is a multi-functional glycoprotein that plays a crucial role in lysosomal function, cell signaling, and the maintenance of tissue homeostasis. It is the precursor of four individual saposins that assist in the hydrolysis of sphingolipids, crucial for lipid metabolism and cellular communication. Mutations in the PSAP gene have been linked to several lysosomal storage diseases, underscoring its importance in human health. Recent studies have highlighted the potential therapeutic applications of PSAP and its derived peptides in neuroprotection, regenerative medicine, and inflammation regulation. The recombinant expression of PSAP and its saposin derivatives in various host systems has become a focus of research, as it allows for the study of their biological functions and the development of novel treatments. Advances in recombinant protein technology facilitate the production of active and soluble forms of PSAP, enabling researchers to explore its mechanisms of action and therapeutic potential in disease models. This research not only enhances our understanding of the protein's role in cellular processes but also opens new avenues for the development of therapies targeting lysosomal dysfunction and neurodegenerative diseases.
