Analytical Data
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Gene name
NCKIPSD
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简介
NCKIPSD Protein, vital for stress fiber formation induced by active DRF1, stimulates N-WASP-induced ARP2/3 complex activation, independent of CDC42. Implicated in sarcomere maintenance, myofibril assembly, actin polymerization, and angiogenesis. Associates with vimentin, desmin, NCK, GRB2, and interacts with Helicobacter pylori toxin vacA, FHOD1, FASLG, and TMIGD2, showcasing its diverse cellular interactions. NCKIPSD Protein, Human (His) is the recombinant human-derived NCKIPSD protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
NCK-interacting protein with SH3 domain; VIP54; WISH; AF3P21; SPIN90
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9NZQ3-3
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Expression Region
M1-T244
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AA Sequence
MYRALYAFRSAEPNALAFAAGETFLVLERSSAHWWLAARARSGETGYVPPAYLRRLQGLEQDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGPSASSVAVMTSSTSDHHLDAAAARQPNGVCRAGFERQHSLPSSEHLGADGGLYQIPPQPRRAAPTTPPPPVKRRDREALMASGSGGHNTMPSGGNSVSSGSSVSSTSLDTLYTSSSPSEPGSSCSPTPPPVPRRGT
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Protein Length
Partial
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Molecular Weight
33-45 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NCKIPSD (NCK-interacting protein, SH3 domain containing) is a protein implicated in various cellular processes, including cell signaling, cytoskeletal organization, and synaptic function. Its role in the context of neurodevelopment and neuroplasticity has drawn significant attention, particularly in relation to synapse formation and maintenance. Research on NCKIPSD has revealed its involvement in the interaction with multiple signaling pathways, especially those pertaining to the actin cytoskeleton and neuronal communication. The study of its recombinant forms has provided insights into its functional mechanisms, facilitating the exploration of its potential roles in neurodegenerative diseases and other neurological disorders. Recombination technology allows for the expression and purification of NCKIPSD, enabling in-depth analysis of its structural and functional properties. By understanding how NCKIPSD operates at the molecular level, researchers aim to uncover its contributions to synaptic integrity and the overall health of the nervous system, paving the way for therapeutic strategies that could address conditions linked to synaptic dysfunction. Furthermore, the investigation of NCKIPSD's interactions with other cellular components may reveal novel pathways and targets for drug development, highlighting its significance in both basic research and clinical applications. As such, the study of NCKIPSD and its recombinant protein forms stands as a promising avenue for advancing our understanding of the complex interplay between protein interactions and neuronal health.
