Analytical Data
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Gene name
beta-Catenin
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简介
β-Catenin is a key component of canonical Wnt signaling, which dynamically controls cellular processes. In the absence of Wnt, it forms a complex leading to phosphorylation and degradation. beta-Catenin Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived beta-Catenin protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
beta-Catenin; Catenin beta-1; CTNNB; CTNNB1
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Species
Mouse
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q02248
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Expression Region
M1-L781
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Protein Length
Full Length
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Molecular Weight
115 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Beta-catenin is a crucial component of the Wnt signaling pathway, which plays a significant role in cell-cell adhesion and the regulation of gene transcription associated with cell proliferation and differentiation. Dysregulation of beta-catenin has been linked to various diseases, particularly cancer, making it a focal point in research aimed at understanding tumorigenesis and potential therapeutic interventions. The study of recombinant beta-catenin proteins has gained importance as these proteins can be used to investigate the molecular mechanisms governing Wnt signaling and its implications in developmental biology and oncogenesis. By producing recombinant beta-catenin in various systems, researchers can explore its functional properties, interaction with other cellular partners, and the effects of post-translational modifications. This research provides insights into the differential roles beta-catenin plays in normal physiology versus pathological states, thereby enhancing our knowledge of targeted therapies in cancers where beta-catenin is aberrantly activated. Additionally, such studies facilitate the development of small molecules or biologics that can modulate the Wnt/beta-catenin pathway, offering promising avenues for therapeutic exploitation in diseases characterized by its dysregulation. The understanding gained from beta-catenin recombinant protein research is vital for the advancement of precision medicine and the design of innovative treatment strategies tailored to target Wnt-related diseases.
