Analytical Data
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Gene name
CDK2-CCND1
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简介
CDK2 is an important serine/threonine protein kinase that plays a critical regulatory role in the cell cycle, especially during meiosis. It phosphorylates various substrates (CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2) and coordinates centrosome duplication and initiation of DNA synthesis at the G1-S transition. CDK2-CCND1 Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK2-CCND1, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.
- Application
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Alternative Names
CDK2; cyclin-dependent kinase 2; p33 protein kinase; cell devision kinase 2; cdc2-related protein kinase; cell division protein kinase 2; p33(CDK2)
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST;N-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P24941-1 (M1-L298 )&P24385
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Expression Region
P24941-1 (M1-L298 )&P24385 (M1-I295 )
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Molecular Weight
60.5 kDa&36.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cyclin-dependent kinase 2 (CDK2) and cyclin D1 (CCND1) play crucial roles in cell cycle regulation, particularly in the transition from the G1 phase to the S phase. CDK2, when activated by binding to cyclins, facilitates the phosphorylation of key substrates that drive cell cycle progression. CCND1, a member of the cyclin protein family, partners with CDK4/6 and CDK2 to regulate the cell cycle checkpoints. Dysregulation of CDK2 and CCND1 is implicated in various cancers, making them attractive targets for therapeutic intervention. Understanding the interactions and functional dynamics of CDK2-CCND1, therefore, is essential in elucidating cancer pathogenesis and developing potential treatment strategies. Researchers have been investigating the molecular mechanisms that govern the CDK2-CCND1 complex to identify novel inhibitors or modulators that could effectively disrupt cancer cell proliferation. Additionally, studies focusing on the post-translational modifications of CDK2 and CCND1, as well as their interactions with other regulatory proteins, have provided insights into their roles in normal and malignant cell biology. This knowledge could pave the way for new diagnostic markers and targeted therapies aimed at cancers associated with aberrant CDK2 and CCND1 activity.
