Analytical Data
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Gene name
SNAP25
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简介
SNAP25 is a key t-SNARE in neurotransmitter release, regulating synaptic function and plasma membrane recycling. It cooperates with CENPF to affect vesicle docking and membrane fusion. SNAP25 Protein, Human (His) is the recombinant human-derived SNAP25 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Synaptosomal-associated protein 25; SNAP-25; SUP; SNAP
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P60880
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Expression Region
M1-G206
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Protein Length
Full Length
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Molecular Weight
28 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SNAP25, or Synaptosomal-Associated Protein 25 kDa, is a crucial membrane protein that plays a significant role in synaptic transmission. It is a key component of the SNARE (Soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex, which facilitates the fusion of synaptic vesicles with the presynaptic membrane, enabling neurotransmitter release. Research on SNAP25 has garnered attention due to its involvement in various neurological disorders, including schizophrenia, autism, and attention deficit hyperactivity disorder (ADHD). Its function is modulated by post-translational modifications, such as phosphorylation and palmitoylation, which can influence synaptic plasticity and overall neuronal communication. Moreover, SNAP25 has been implicated in toxin-mediated processes, such as the action of botulinum neurotoxins, which cleave SNAP25 and disrupt neurotransmission, leading to paralysis. Understanding the structural and functional dynamics of recombinant SNAP25 is therefore essential for elucidating its role in both healthy and pathological states. Recent advances in protein engineering and crystallography have facilitated the characterization of SNAP25, providing insights into its interactions with other SNARE proteins and cellular machinery. This research not only enhances our understanding of fundamental neurobiology but also holds potential for therapeutic interventions aimed at modulating neurotransmitter release in various psychiatric and neurological conditions. Thus, the study of recombinant SNAP25 continues to be a vital area within neuroscience, bridging molecular mechanisms and potential clinical applications.
