Analytical Data
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Gene name
BAP1
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简介
BAP1 protein deubiquitinates histone H2A and HCFC1, mediating deubiquitination of H2AK119ub1. It regulates cell growth by deubiquitinating HCFC1 chains and inhibits BRCA1 and BARD1 activity. BAP1 also protects itself from cytoplasmic sequestration and acts as a tumor suppressor. BAP1 Protein, Human (His) is the recombinant human-derived BAP1 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
BAP1; Ubiquitin carboxyl-terminal hydrolase BAP1; BRCA1-associated protein 1; Cerebral protein 6
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q92560
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Expression Region
N2-Q729
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BAP1, or BRCA1-associated protein-1, is a key tumor suppressor encoded by the BAP1 gene, which plays a pivotal role in cellular processes such as chromatin remodeling, DNA repair, and cell cycle regulation. Dysregulation or loss of BAP1 function is associated with various malignancies, including malignant mesothelioma, uveal melanoma, and several other cancers, highlighting its significance in tumorigenesis. The protein contains a ubiquitin carboxy-terminal (UBR) domain, a nuclear localization sequence, and several other structural motifs, which facilitate its interaction with multiple protein partners involved in the regulation of gene expression and DNA damage response. Research on recombinant BAP1 has gained momentum as scientists seek to understand its biochemical pathways and interactions that govern tumor suppression. This includes the characterization of its enzymatic activity, particularly its role as a deubiquitinase, which is crucial for modulating the stability and activity of various oncogenic proteins. Furthermore, recombinant BAP1 studies have been instrumental in elucidating its impact on cellular signaling pathways, particularly those involving the Hippo pathway, which is implicated in regulating cell proliferation and survival. Given its critical functions, BAP1 is not only a biomarker for cancer predisposition but also a potential therapeutic target, spurring research efforts to develop BAP1-based strategies for cancer treatment. Understanding the molecular mechanisms mediated by BAP1 and how its dysfunction contributes to tumorigenesis can pave the way for novel diagnostic and therapeutic approaches, ultimately aiming to enhance cancer management and patient outcomes.
