Analytical Data
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Gene name
NHP2
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简介
The NHP2 protein is essential for ribosome biogenesis and telomere maintenance and is a key component of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex. In this complex, NHP2 catalyzes pseudouridylation of rRNA by isomerizing uridine, thereby stabilizing the rRNA conformation with up to 100 pseudouridine residues. NHP2 Protein, Human (His) is the recombinant human-derived NHP2 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
H/ACA ribonucleoprotein complex subunit 2; Nucleolar protein family A member 2; snoRNP protein NHP2; NHP2; NOLA2; NHP2P.
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NX24
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Expression Region
M1-L153
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Protein Length
Full Length
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Molecular Weight
22 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NHP2, a member of the small nucleolar RNA (snoRNA) binding protein family, plays a crucial role in ribosome biogenesis and the regulation of pre-rRNA processing. Its involvement in the maturation of ribosomal RNA makes it essential for proper ribosome function, which is fundamental to cellular protein synthesis. Recent studies have also indicated that NHP2 may be implicated in various cellular processes, including RNA metabolism and splicing, highlighting its potential influence on gene expression and cellular homeostasis. Moreover, dysfunction or altered expression of NHP2 has been linked to various diseases, including certain cancers, underscoring its significance in pathophysiological conditions. The recombinant expression of NHP2 provides a valuable tool for studying its structural and functional properties in vitro. By producing NHP2 as a recombinant protein, researchers aim to elucidate its molecular interactions and mechanisms of action, contributing to a greater understanding of its role in ribosome assembly and its potential as a therapeutic target. Additionally, the study of NHP2 through recombinant protein techniques may offer insights into the development of novel strategies for manipulating ribosome biogenesis in disease states, paving the way for innovative therapeutic approaches. Overall, the investigation of NHP2 recombinant protein continues to be of significant interest within the fields of molecular biology and biochemistry, as it holds promise for advancing our comprehension of RNA-protein interactions and their implications in human health.
