Analytical Data
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Gene name
GEMIN8
- Application
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Alternative Names
GEMIN8; FAM51A1Gem-associated protein 8; Gemin-8; Protein FAM51A1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NWZ8
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Expression Region
1-242aa
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AA Sequence
MAAVKASTSKATRPWYSHPVYARYWQHYHQAMAWMQSHHNAYRKAVESCFNLPWYLPSALLPQSSYDNEAAYPQSFYDHHVAWQDYPCSSSHFRRSGQHPRYSSRIQASTKEDQALSKEEEMETESDAEVECDLSNMEITEELRQYFAETERHREERRRQQQLDAERLDSYVNADHDLYCNTRRSVEAPTERPGERRQAEMKRLYGDSAAKIQAMEAAVQLSFDKHCDRKQPKYWPVIPLKF
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Molecular Weight
55 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GEMIN8 is a pivotal protein within the GEMIN family, known for its critical roles in the biogenesis and function of the spliceosomal machinery, particularly in the context of pre-mRNA splicing and RNA metabolism. Recent studies have highlighted its significance in the assembly of the spliceosome complex and its interaction with small nuclear ribonucleoproteins (snRNPs), which are essential for accurate splicing processes. Genetic mutations or dysregulation of GEMIN8 have been implicated in various neurodegenerative diseases, including Spinal Muscular Atrophy (SMA) and Amyotrophic Lateral Sclerosis (ALS), making it a focal point of research in understanding the molecular mechanisms underlying these conditions. Moreover, GEMIN8's involvement in cellular stress responses and its potential role in maintaining neuronal integrity further underscore its importance in both health and disease. Given these associations, researchers are actively investigating GEMIN8's structural properties and functional dynamics, aiming to elucidate its precise role within the spliceosomal assembly pathway and its contributions to disease pathology. Understanding GEMIN8's mechanisms could provide novel insights into therapeutic strategies for targeting splicing-related disorders, highlighting its potential as a biomarker or a therapeutic target in neurodegenerative disease contexts.
