Analytical Data
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Gene name
GJB5
- Application
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Alternative Names
GJB5; Gap junction beta-5 protein; Connexin-31.1; Cx31.1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95377
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Expression Region
1-273aa
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AA Sequence
MNWSIFEGLLSGVNKYSTAFGRIWLSLVFIFRVLVYLVTAERVWSDDHKDFDCNTRQPGCSNVCFDEFFPVSHVRLWALQLILVTCPSLLVVMHVAYREVQEKRHREAHGENSGRLYLNPGKKRGGLWWTYVCSLVFKASVDIAFLYVFHSFYPKYILPPVVKCHADPCPNIVDCFISKPSEKNIFTLFMVATAAICILLNLVELIYLVSKRCHECLAARKAQAMCTGHHPHGTTSSCKQDDLLSGDLIFLGSDSHPPLLPDRPRDHVKKTIL
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Molecular Weight
55.77 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GJB5, also known as Gap Junction Protein Beta 5, is a member of the connexin family, which plays a crucial role in cellular communication by forming gap junctions that facilitate the direct transfer of ions and small molecules between neighboring cells. Mutations in the GJB5 gene have been implicated in various human diseases, particularly skin disorders such as ectodermal dysplasias and hearing loss. The study of GJB5 recombinant protein is significant as it helps in understanding the structure-function relationships of gap junctions and their roles in maintaining cellular homeostasis. By producing and characterizing GJB5 recombinant protein, researchers aim to elucidate the molecular mechanisms underlying its function and the pathophysiology of diseases associated with its mutation. Furthermore, studying this protein can lead to insights into potential therapeutic strategies for conditions arising from impaired cell-to-cell communication. Given the essential nature of gap junctions in tissue integrity and intercellular signaling, the exploration of GJB5 and its interactions is vital for future biomedical applications, including drug development and gene therapy targeting connexin-related pathologies.
