Analytical Data
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Gene name
GKAP1
- Application
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Alternative Names
cGMP dependent protein kinase anchoring protein 42kDa; cGMP dependent protein kinase anchoring protein of 42 kDa; cGMP-dependent protein kinase-anchoring protein of 42 kDa; FKSG21; G kinase anchoring protein 1; G kinase-anchoring protein 1; gkap1; GKAP1_HUMAN; GKAP42; Protein kinase anchoring protein GKAP42
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5VSY0
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Expression Region
1-366aa
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AA Sequence
MASAVLSSVPTTASRFALLQVDSGSGSDSEPGKGKGRNTGKSQTLGSKSTTNEKKREKRRKKKEQQQSEANELRNLAFKKIPQKSSHAVCNAQHDLPLSNPVQKDSREENWQEWRQRDEQLTSEMFEADLEKALLLSKLEYEEHKKEYEDAENTSTQSKVMNKKDKRKNHQGKDRPLTVSLKDFHSEDHISKKTEELSSSQTLSHDGGFFNRLEDDVHKILIREKRREQLTEYNGTDNCTAHEHNQEVVLKDGRIERLKLELERKDAEIQKLKNVITQWEAKYKEVKARNAQLLKMLQEGEMKDKAEILLQVDESQSIKNELTIQVTSLHAALEQERSKVKVLQAELAKYQGGRKGKRNSESDQCR
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Molecular Weight
66 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GKAP1, or Grb2-associated binding protein 1, is a pivotal scaffolding protein that plays a critical role in synaptic signaling and the regulation of actin dynamics in neurons. As a member of the PSD-95/SAP90 family, GKAP1 interacts with various proteins, including neurotransmitter receptors and cytoskeletal elements, thereby facilitating the formation and stabilization of synaptic complexes. This functionality is crucial for synaptic plasticity, which underlies learning and memory processes. Research on recombinant GKAP1 protein has gained momentum in recent years, particularly in elucidating its involvement in neurodevelopmental and neuropsychiatric disorders. Dysregulation of GKAP1 expression has been implicated in conditions such as schizophrenia, autism spectrum disorders, and cognitive deficits, prompting scientists to explore its potential as a therapeutic target. By analyzing the biophysical and biochemical properties of recombinant GKAP1, researchers aim to understand its molecular mechanisms of action and the pathways it influences. Additionally, investigations into GKAP1 interactions with partner proteins and ligands through structural biology techniques, such as X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, are providing insights into its role in cellular signaling networks. Ultimately, the study of recombinant GKAP1 not only enriches our understanding of synaptic biology but also holds promise for developing innovative strategies for treating neurological disorders linked to synaptic dysfunction.
