Analytical Data
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Gene name
AchE
- Application
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Alternative Names
AchE；ACHRE；Acetylcholine receptor subunit epsilon
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P22303
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Expression Region
1-640aa
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AA Sequence
MRPPQCLLHTPSLASPLLLLLVWLLGGGVGAEGREDAELLVTVRGGRLRG IRLKTPGGPVSAFLGIPFAEPPMGPRRFLPPEPKQPWSGVVDATTFQSVC YQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVIVWIY GGGFYSGASSLDVYDGRFLVQAERTVLVSMNYRVGAFGFLALPGSREAPG NVGLLDQRLALQWVQENVAAFGGDPTSVTLFGESAGAASVGMHLLSPPSR GLFHRAVLQSGAPNGPWATVGMGEARRRATQLAHLVGCPPGGTGGNDTEL VACLRTRPAQVLVNHEWHVLPQESVFRFSFVPVVDGDFLSDTPEALINAG DFHGLQVLVGVVKDEGSYFLVYGAPGFSKDNESLISRAEFLAGVRVGVPQ VSDLAAEAVVLHYTDWLHPEDPARLREALSDVVGDHNVVCPVAQLAGRLA AQGARVYAYVFEHRASTLSWPLWMGVPHGYEIEFIFGIPLDPSRNYTAEE KIFAQRLMRYWANFARTGDPNEPRDPKAPQWPPYTAGAQQYVSLDLRPLE VRRGLRAQACAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKN QFDTTASRIAAQTCDPGGTPMSSAPPGPLAVYTIYFRAGL
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Molecular Weight
97 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Acetylcholinesterase (AChE) is a vital enzyme responsible for the hydrolysis of the neurotransmitter acetylcholine in the synaptic cleft, playing a crucial role in synaptic transmission and muscle contraction. Its importance is underscored by its involvement in various physiological processes and its implications in neurodegenerative diseases, such as Alzheimer's disease, where its activity is significantly altered. Research into recombinant AChE proteins has gained momentum as scientists explore their potential applications in diagnostics, therapeutic interventions, and as tools in biochemical studies. Advances in molecular cloning and expression systems have enabled the production of recombinant AChE with desired properties, such as enhanced stability, specificity, and activity. These engineered proteins not only facilitate a deeper understanding of AChE's structure-function relationship but also provide insights into the mechanisms underlying various pathologies linked to its dysfunction. Additionally, recombinant AChE has potential utility in developing antidotes against organophosphate poisoning, given its ability to effectively regenerate neurotransmission. Therefore, the study of AChE recombinant proteins is a rapidly evolving field that bridges fundamental biochemistry and potential clinical applications, addressing pressing challenges in neurobiology and toxicology.
