Analytical Data
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Gene name
HTT
- Application
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Alternative Names
HTT；HD；IT15；Huntingtin
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P42858
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Expression Region
全长
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AA Sequence
full
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HTT (Huntingtin) is a protein encoded by the HTT gene, which plays a critical role in neuronal function and development. Research into HTT has gained significant attention due to its association with Huntington's disease, a hereditary neurodegenerative disorder characterized by motor dysfunction, cognitive decline, and psychiatric symptoms. The pathogenesis of Huntington's disease is linked to the expansion of CAG repeats in the HTT gene, leading to the production of a mutant form of the protein. This mutant HTT is known to aggregate within neurons, disrupting cellular functions and triggering apoptotic pathways. Studies have focused on elucidating the molecular mechanisms by which mutant HTT exerts its toxic effects, exploring its interactions with cellular machinery, including protein aggregation pathways, mitochondrial function, and synaptic integrity. Furthermore, researchers are investigating potential therapeutic strategies aimed at reducing mutant HTT levels, such as gene silencing approaches and small molecules that can modulate its activity. Understanding the structure and function of HTT is vital for developing effective interventions to combat the progression of Huntington's disease, providing insight into not only its pathophysiology but also broader implications for neurodegeneration.
