Analytical Data
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Gene name
SDHB
- Application
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Alternative Names
SDHB；SDH；SDH1；Succinate dehydrogenase [ubiquinone] iron-sulfur subunit. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P21912
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Expression Region
29-280aa
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AA Sequence
AQ TAAATAPRIK KFAIYRWDPD KAGDKPHMQT YEVDLNKCGP MVLDALIKIK NEVDSTLTFR RSCREGICGS CAMNINGGNT LACTRRIDTN LNKVSKIYPL PHMYVIKDLV PDLSNFYAQY KSIEPYLKKK DESQEGKQQY LQSIEEREKL DGLYECILCA CCSTSCPSYW WNGDKYLGPA VLMQAYRWMI DSRDDFTEER LAKLQDPFSL YRCHTIMNCT RTCPKGLNPG KAIAEIKKMM ATYKEKKASV
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SDHB (Succinate Dehydrogenase B) is a crucial enzyme in the mitochondrial respiratory chain, playing a significant role in cellular respiration and energy production. It is part of the larger succinate dehydrogenase complex, which participates in both the tricarboxylic acid (TCA) cycle and the electron transport chain. Research on SDHB has gained prominence due to its association with genetic disorders, particularly hereditary pheochromocytomas and paragangliomas, as mutations in the SDHB gene can lead to these conditions. Additionally, the dysfunction of SDHB has been linked to various types of cancer, including familial gastrointestinal stromal tumors and certain forms of kidney cancer, emphasizing its importance in tumorigenesis. The recombinant expression of SDHB protein has become essential for studying its functional characteristics, understanding the molecular mechanisms underlying its role in metabolism, and exploring its implications in disease. By producing SDHB in heterologous systems, researchers can investigate the enzyme's biochemical properties, assess the impact of specific mutations, and develop potential therapeutic strategies. This research not only enhances our understanding of metabolic diseases but also opens avenues for targeted treatments in related cancers, making SDHB a vital focus in both basic and clinical research.
