Analytical Data
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Gene name
ULK3
- Application
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Alternative Names
DKFZP434C131; FLJ90566; Serine/threonine-Protein kinase ULK3; Ulk3; ULK3_HUMAN; unc 51 like kinase 3 (C. elegans); Unc-51-like kinase 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6PHR2
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Expression Region
1-472 aa
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AA Sequence
MAGPGWGPPR LDGFILTERL GSGTYATVYK AYAKKDTREV VAIKCVAKKS LNKASVENLL TEIEILKGIR HPHIVQLKDF QWDSDNIYLI MEFCAGGDLS RFIHTRRILP EKVARVFMQQ LASALQFLHE RNISHLDLKP QNILLSSLEK PHLKLADFGF AQHMSPWDEK HVLRGSPLYM APEMVCQRQY DARVDLWSMG VILYEALFGQ PPFASRSFSE LEEKIRSNRV IELPLRPLLS RDCRDLLQRL LERDPSRRIS FQDFFAHPWV DLEHMPSGES LGRATALVVQ AVKKDQEGDS AAALSLYCKA LDFFVPALHY EVDAQRKEAI KAKVGQYVSR AEELKAIVSS SNQALLRQGT SARDLLREMA RDKPRLLAAL EVASAAMAKE EAAGGEQDAL DLYQHSLGEL LLLLAAEPPG RRRELLHTEV QNLMARAEYL KEQVKMRESR WEADTLDKEG LSESVRSSCT LQ
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Molecular Weight
53.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ULK3 (Unc-51 Like Autophagy Activating Kinase 3) is a serine/threonine kinase that plays a crucial role in the regulation of autophagy, a cellular process involved in degrading and recycling cellular components. The study of ULK3 has gained significant attention due to its implications in various physiological and pathological contexts, including cancer, neurodegenerative diseases, and metabolic disorders. As a member of the ULK family, ULK3 is activated in response to cellular stressors and nutrient availability, thus serving as an important regulator in the autophagy pathway. Understanding the molecular mechanisms underlying ULK3’s function may unveil new therapeutic targets for diseases characterized by dysregulated autophagy. Recent research has focused on the purification and characterization of ULK3 recombinant protein, which provides a valuable tool for studying its enzymatic activity, substrate specificity, and interaction with other proteins within the autophagy machinery. The generation of ULK3 recombinant protein allows researchers to investigate its role in autophagy modulation and explore potential avenues for drug development aimed at enhancing or inhibiting autophagy in various disease contexts. Moreover, studying the post-translational modifications and regulatory mechanisms of ULK3 could yield insights into how cellular signaling pathways influence autophagy and how these processes are altered in diseases. Overall, research on ULK3 recombinant protein is expected to contribute significantly to our understanding of autophagy dynamics and its implications for human health.
