Analytical Data
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Gene name
UHRF1
- Application
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Alternative Names
Hypothetical Protein LOC23074; UH1BL_HUMAN; UHRF1 binding Protein 1 like; UHRF1-binding Protein 1-like; UHRF1BP1L
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A0JNW5
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Expression Region
1-806 aa
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AA Sequence
MGVFAVPPLSSDTMWIQVRTMDGRQTHTVDSLSRLTKVEELRRKIQELFHVEPGLQRLFYRGKQMEDGHTLFDYEVRLNDTIQLLVRQSLVLPHSTKERDSELSDTDSGCCLGQSESDKSSTHGEAAAETDSRPADEDMWDETELGLYKVNEYVDARDTNMGAWFEAQVVRVTRKAPSRDEPCSSTSRPALEEDVIYHVKYDDYPENGVVQMNSRDVRARARTIIKWQDLEVGQVVMLNYNPDNPKERGFWYDAEISRKRETRTARELYANVVLGDDSLNDCRIIFVDEVFKIERPGEGSPMVDNPMRRKSGPSCKHCKDDVNRLCRVCACHLCGGRQDPDKQLMCDECDMAFHIYCLDPPLSSVPSEDEWYCPECRNDASEVVLAGERLRESKKKAKMASATSSSQRDWGKGMACVGRTKECTIVPSNHYGPIPGIPVGTMWRFRVQVSESGVHRPHVAGIHGRSNDGAYSLVLAGGYEDDVDHGNFFTYTGSGGRDLSGNKRTAEQSCDQKLTNTNRALALNCFAPINDQEGAEAKDWRSGKPVRVVRNVKGGKNSKYAPAEGNRYDGIYKVVKYWPEKGKSGFLVWRYLLRRDDDEPGPWTKEGKDRIKKLGLTMQYPEGYLEALANREREKENSKREEEEQQEGGFASPRTGKGKWKRKSAGGGPSRAGSPRRTSKKTKVEPYSLTAQQSSLIREDKSNAKLWNEVLASLKDRPASGSPFQLFLSKVEETFQCICCQELVFRPITTVCQHNVCKDCLDRSFRAQVFSCPACRYDLGRSYAMQVNQPLQTVLNQLFPGYGNGR
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Molecular Weight
115.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
UHRF1 (Ubiquitin-like with PHD and RING finger domains 1) is an essential protein involved in epigenetic regulation and plays a critical role in maintaining DNA methylation during cell division. It acts as a bridge between DNA methyltransferases and the histone modification machinery, thereby linking DNA methylation with histone modification. Research has shown that UHRF1 is not only crucial for the proper propagation of DNA methylation patterns but also is implicated in various cellular processes, including the regulation of gene expression and maintenance of genomic integrity. Dysregulation of UHRF1 has been associated with several types of cancers and various diseases, making it a significant target for therapeutic intervention. Recombinant UHRF1 protein studies facilitate the understanding of its structure-function relationship and its role in epigenetic modifications. By producing UHRF1 in vitro, researchers can rigorously examine its biochemical properties, interaction with other epigenetic modulators, and its impact on both normal cellular processes and disease states. This research not only provides insights into the molecular mechanisms underpinning UHRF1's function but also opens avenues for the development of novel epigenetic therapies. Overall, the study of recombinant UHRF1 is pivotal for elucidating its role in epigenetic regulation and its potential implications in cancer biology and therapy.
