Analytical Data
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基因名
APPL1
- Application
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别名
APPL1；DIP13B；DCC-interacting Protein 13-beta
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UKG1
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表达区间
5-377aa
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氨基酸序列
DKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLANIGTSVQNVRREMDSDIETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQI
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分子量
70.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
APPL1 (adaptor protein containing PH and LE domains) is a key regulatory protein involved in various cellular processes, including endocytosis, signal transduction, and cytoskeletal dynamics. It serves as an adapter that links different signaling pathways, particularly those related to insulin signaling and glucose homeostasis, making it a significant player in metabolic regulation. Research has shown that APPL1 interacts with multiple proteins, such as AKT and Rab5, facilitating intracellular trafficking and influencing cellular responses to environmental stimuli. Dysregulation of APPL1 has been implicated in several metabolic disorders, including obesity and type 2 diabetes. The study of recombinant APPL1 protein is crucial for understanding its structural and functional properties, which can lead to insights into its role in metabolic pathways. By producing and characterizing recombinant APPL1, researchers aim to elucidate its interaction mechanisms and regulatory functions within cells. This research not only contributes to basic science but also has potential therapeutic implications, as targeting APPL1-related pathways could offer new strategies for treating metabolic diseases. Overall, the investigation of APPL1 serves as an important avenue for uncovering molecular targets for interventions in metabolic disorders, thereby advancing our understanding of cellular metabolism and disease progression.
