Analytical Data
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Gene name
TYW3
- Application
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Alternative Names
TYW3; C1orf171; tRNA wybutosine-synthesizing Protein 3 homolog; tRNA-yW-synthesizing Protein 3; EC 2.1.1.282; tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6IPR3
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Expression Region
1-259 aa
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AA Sequence
MDRSAEFRKW KAQCLSKADL SRKGSVDEDV VELVQFLNMR DQFFTTSSCA GRILLLDRGI NGFEVQKQNC CWLLVTHKLC VKDDVIVALK KANGDATLKF EPFVLHVQCR QLQDAQILHS MAIDSGFRNS GITVGKRGKT MLAVRSTHGL EVPLSHKGKL MVTEEYIDFL LNVANQKMEE NKKRIERFYN CLQHALERET MTNLHPKIKE KNNSSYIHKK KRNPEKTRAQ CITKESDEEL ENDDDDDLGI NVTIFPEDY
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Molecular Weight
29.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The research on TYW3 recombinant protein has gained significant attention in the field of molecular biology and biochemistry due to its crucial role in the modification and maturation of tRNA. TYW3, a member of the trypanthin family, is involved in the addition of the nucleoside 2-thiouridine to specific tRNA molecules, which is essential for proper tRNA function and the accuracy of protein translation. Deficiency or malfunction of TYW3 can lead to various diseases, including mitochondrial dysfunction and defects in protein synthesis that may contribute to cancer and neurological disorders. Studies on TYW3 recombinant protein aim to elucidate its biochemical pathways, enhance our understanding of its mechanism of action, and explore its potential therapeutic applications. The production of TYW3 in a recombinant form allows for detailed studies of its structure and function, providing valuable insights into its role in cellular processes. Furthermore, the ability to manipulate this protein may lead to innovative approaches in the development of treatments for conditions associated with tRNA modification deficiencies. Overall, understanding TYW3 and its properties presents a promising avenue for advancing the field of genetic research and improving therapeutic strategies.
