Analytical Data
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Gene name
TWISTNB
- Application
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Alternative Names
DNA directed RNA polymerase I subunit RPA43; DNA-directed RNA polymerase I subunit RPA43; RPA43; RPA43_HUMAN; TWIST neighbor; Twist neighbor Protein; Twistnb
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q3B726
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Expression Region
1-338 aa
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AA Sequence
MAAGCSEAPR PAAASDGSLV GQAGVLPCLE LPTYAAACAL VNSRYSCLVA GPHQRHIALS PRYLNRKRTG IREQLDAELL RYSESLLGVP IAYDNIKVVG ELGDIYDDQG HIHLNIEADF VIFCPEPGQK LMGIVNKVSS SHIGCLVHGC FNASIPKPEQ LSAEQWQTME INMGDELEFE VFRLDSDAAG VFCIRGKLNI TSLQFKRSEV SEEVTENGTE EAAKKPKKKK KKKDPETYEV DSGTTKLADD ADDTPMEESA LQNTNNANGI WEEEPKKKKK KKKHQEVQDQ DPVFQGSDSS GYQSDHKKKK KKRKHSEEAE FTPPLKCSPK RKGKSNFL
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Molecular Weight
37.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TWISTNB is a recombinant protein that has garnered significant attention in the field of biochemistry and molecular biology due to its unique structural and functional properties. Derived from the TWIST family of helix-loop-helix transcription factors, TWISTNB plays a crucial role in various biological processes, including cell differentiation, proliferation, and apoptosis. The study of TWISTNB is particularly relevant in cancer research, as it has been implicated in the regulation of epithelial-mesenchymal transition (EMT), a process that allows cancer cells to acquire migratory and invasive capabilities. Through reprogramming cellular pathways, TWISTNB can influence tumor progression and metastasis, making it a potential target for therapeutic intervention. Additionally, the expression and activity of TWISTNB are often altered in various cancers, thus serving as a biomarker for disease prognosis. Advances in recombinant DNA technology have enabled the production of TWISTNB in vitro, facilitating detailed investigations into its mechanisms of action and interactions with other cellular components. This foundational research not only enhances our understanding of cancer biology but also paves the way for the development of novel therapeutic strategies aimed at inhibiting TWISTNB-related pathways, thereby holding promise for improved cancer treatment outcomes. The ongoing exploration of TWISTNB's properties and functions continues to reveal its pivotal role in modern biomedical research, underscoring the importance of recombinant protein studies in addressing complex health challenges.
