Analytical Data
-
Gene name
TTC8
- Application
-
Alternative Names
Bardet Biedl syndrome 8 Protein; Bardet Biedl syndrome type 8; Bardet-Biedl syndrome 8 Protein; BBS8; RP51; Tetratricopeptide repeat domain 8; Tetratricopeptide repeat Protein 8; TPR repeat Protein 8; TTC 8; Ttc8; TTC8_HUMAN
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q8TAM2
-
Expression Region
1-541 aa
-
AA Sequence
MSSEMEPLLL AWSYFRRRKF QLCADLCTQM LEKSPYDQEP DPELPVHQAA WILKARALTE MVYIDEIDVD QEGIAEMMLD ENAIAQVPRP GTSLKLPGTN QTGGPSQAVR PITQAGRPIT GFLRPSTQSG RPGTMEQAIR TPRTAYTARP ITSSSGRFVR LGTASMLTSP DGPFINLSRL NLTKYSQKPK LAKALFEYIF HHENDVKTIH LEDVVLHLGI YPFLLRNKNH IEKNALDLAA LSTEHSQYKD WWWKVQIGKC YYRLGMYREA EKQFKSALKQ QEMVDTFLYL AKVYVSLDQP VTALNLFKQG LDKFPGEVTL LCGIARIYEE MNNMSSAAEY YKEVLKQDNT HVEAIACIGS NHFYSDQPEI ALRFYRRLLQ MGIYNGQLFN NLGLCCFYAQ QYDMTLTSFE RALSLAENEE EAADVWYNLG HVAVGIGDTN LAHQCFRLAL VNNNNHAEAY NNLAVLEMRK GHVEQARALL QTASSLAPHM YEPHFNFATI SDKIGDLQRS YVAAQKSEAA FPDHVDTQHL IKQLRQHFAM L
-
Molecular Weight
61.5 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TTC8, also known as Tetra-Tricopeptide Repeat-containing Protein 8, is a member of the TTC family of proteins characterized by the presence of multiple tetratricopeptide repeats (TPRs). These TPRs play critical roles in protein-protein interactions, which are essential for various cellular processes. Research into TTC8 has gained attention due to its association with Bardet-Biedl syndrome (BBS), a genetic disorder characterized by obesity, retinal degeneration, polydactyly, and renal abnormalities. Mutations in the TTC8 gene have been linked to a subset of BBS cases, suggesting that TTC8 is crucial for ciliary function and the regulation of intracellular signaling pathways. Recent studies have focused on elucidating the molecular mechanisms underlying TTC8's role in ciliogenesis and its impact on the pathophysiology of ciliary-related diseases. Investigating the recombinant form of TTC8 enables researchers to better understand its structure-function relationships and potential interactions with other ciliary proteins. Furthermore, the expression and purification of TTC8 recombinant protein provides valuable insights into its functional mechanisms, paving the way for potential therapeutic interventions for BBS and related disorders. Overall, the study of TTC8 is significant not only for advancing our knowledge of ciliary biology but also for its implications in understanding the genetic basis of complex human diseases.
