Analytical Data
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Gene name
MAN1A1
- Application
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Alternative Names
MAN1A1；Mannosyl-oligosaccharide 1.2-alpha-mannosidase IA
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P33908
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Expression Region
1-653aa
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AA Sequence
MPVGGLLPLFSSPAGGVLGGGLGGGGGRKGSGPAALRLTEKFVLLLVFSAFITLCFGAIFFLPDSSKLLSGVLFHSSPALQPAADHKPGPGARAEDAAEGRARRREEGAPGDPEAALEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKKKVAQDQLRDKAPFRGLPPVDFVPPIGVESREPADAAIREKRAKIKEMMKHAWNNYKGYAWGLNELKPISKGGHSSSLFGNIKGATIVDALDTLFIMEMKHEFEEAKSWVEENLDFNVNAEISVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKSGIGRNWPWASGGSSILAEFGTLHLEFMHLSHLSGNPIFAEKVMNIRTVLNKLEKPQGLYPNYLNPSSGQWGQHHVSVGGLGDSFYEYLLKAWLMSDKTDLEAKKMYFDAVQAIETHLIRKSSSGLTYIAEWKGGLLEHKMGHLTCFAGGMFALGADAAPEGMAQHYLELGAEIARTCHESYNRTFMKLGPEAFRFDGGVEAIATRQNEKYYILRPEVMETYMYMWRLTHDPKYRKWAWEAVEALENHCRVNGGYSGLRDVYLLHESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNSEAHLLPILPKDKKEVEIREE
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Molecular Weight
72.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MAN1A1, or Mannosidase 1A1, is an essential enzyme involved in the processing of N-linked glycoproteins during the glycosylation pathway, which is crucial for proper protein folding and function. Its catalytic role in trimming mannose residues in glycoproteins ensures the generation of correctly folded proteins that are essential for various biological processes. The study of MAN1A1 has gained significant attention due to its implications in various diseases, including cancer and neurodegenerative disorders, where abnormal glycosylation patterns are often observed. Recombinant MAN1A1 proteins are increasingly being produced for a deeper understanding of its structure-function relationship and potential therapeutic applications. Through studies involving these recombinant proteins, researchers aim to elucidate the underlying mechanisms of glycoprotein biosynthesis and its regulation, potentially leading to the development of novel strategies for disease treatment and biomarker identification. Moreover, understanding the enzyme's activity can aid in the design of specific inhibitors that could modulate glycosylation processes, thereby opening new avenues in pharmaceutical development. The recombinant expression of MAN1A1 also facilitates the exploration of its interactions with other cellular components, thereby providing insights into its broader biological roles.
