Analytical Data
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Gene name
ZNF449
- Application
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Alternative Names
FLJ23614; OTTHUMP00000024072; Zinc finger and SCAN domain containing Protein 19; Zinc finger and SCAN domain-containing Protein 19; Zinc finger Protein 449; ZN449_HUMAN; ZNF449; ZSCAN19
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6P9G9
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Expression Region
1-518 aa
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AA Sequence
MAVALGCAIQASLNQGSVFQEYDTDCEVFRQRFRQFQYREAAGPHEAFNKLWELCCQWLKPKMRSKEQILELLVLEQFLTILPTEIETWVREHCPENRERVVSLIEDLQRELEIPEQQVDMHDMLLEELAPVGTAHIPPTMHLESPALQVMGPAQEAPVAEAWIPQAGPPELNYGATGECQNFLDPGYPLPKLDMNFSLENREEPWVKELQDSKEMKQLLDSKIGFEIGIENEEDTSKQKKMETMYPFIVTLEGNALQGPILQKDYVQLENQWETPPEDLQTDLAKLVDQQNPTLGETPENSNLEEPLNPKPHKKKSPGEKPHRCPQCGKCFARKSQLTGHQRIHSGEEPHKCPECGKRFLRSSDLYRHQRLHTGERPYECTVCKKRFTRRSHLIGHQRTHSEEETYKCLECGKSFCHGSSLKRHLKTHTGEKPHRCHNCGKSFSRLTALTLHQRTHTEERPFKCNYCGKSFRQRPSLVIHLRIHTGEKPYKCTHCSKSFRQRAGLIMHQVTHFRGLI
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Molecular Weight
82.72 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ZNF449 (Zinc Finger Protein 449) is a member of the zinc finger protein family, which is characterized by the presence of zinc finger motifs that facilitate DNA binding and gene regulation. This protein has garnered significant interest due to its potential role in various biological processes and its implications in disease mechanisms, particularly in cancers and developmental disorders. Previous studies suggest that ZNF449 may function as a transcriptional regulator, influencing gene expression patterns that are critical for cell growth and differentiation. Additionally, its involvement in chromatin remodeling and epigenetic regulation places it at the center of research aimed at understanding the complexities of gene regulation and cellular responses to environmental stimuli. The recombinant expression of ZNF449 allows for detailed studies of its structural and functional properties, enabling researchers to elucidate its specific interactions with DNA and other regulatory proteins. Understanding the biochemical characteristics of ZNF449, including its binding affinities, stability, and folding behavior, is crucial for exploring its role in cellular pathways and potential therapeutic targets. As the field of molecular biology continues to evolve, the study of ZNF449 and its recombinant protein form represents a promising avenue for uncovering new insights into gene regulation and the development of innovative strategies for disease intervention. Overall, the investigation of ZNF449 recombinant protein is an essential step towards deciphering the complexities of gene regulatory networks and their implications in health and disease.
