Analytical Data
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基因名
ZNF42
- Application
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别名
Myeloid zinc finger 1; MZF 1; MZF-1; MZF1; MZF1_HUMAN; MZF1B; Zfp98; Zinc finger and SCAN domain containing Protein 6 ; Zinc finger and SCAN domain-containing Protein 6; Zinc finger Protein 42; ZNF42; ZSCAN6
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P28698
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表达区间
1-734 aa
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氨基酸序列
MRPAVLGSPD RAPPEDEGPV MVKLEDSEEE GEAALWDPGP EAARLRFRCF RYEEATGPQE ALAQLRELCR QWLRPEVRSK EQMLELLVLE QFLGALPPEI QARVQGQRPG SPEEAAALVD GLRREPGGPR RWVTVQVQGQ EVLSEKMEPS SFQPLPETEP PTPEPGPKTP PRTMQESPLG LQVKEESEVT EDSDFLESGP LAATQESVPT LLPEEAQRCG TVLDQIFPHS KTGPEGPSWR EHPRALWHEE AGGIFSPGFA LQLGSISAGP GSVSPHLHVP WDLGMAGLSG QIQSPSREGG FAHALLLPSD LRSEQDPTDE DPCRGVGPAL ITTRWRSPRG RSRGRPSTGG GVVRGGRCDV CGKVFSQRSN LLRHQKIHTG ERPFVCSECG RSFSRSSHLL RHQLTHTEER PFVCGDCGQG FVRSARLEEH RRVHTGEQPF RCAECGQSFR QRSNLLQHQR IHGDPPGPGA KPPAPPGAPE PPGPFPCSEC RESFARRAVL LEHQAVHTGD KSFGCVECGE RFGRRSVLLQ HRRVHSGERP FACAECGQSF RQRSNLTQHR RIHTGERPFA CAECGKAFRQ RPTLTQHLRV HTGEKPFACP ECGQRFSQRL KLTRHQRTHT GEKPYHCGEC GLGFTQVSRL TEHQRIHTGE RPFACPECGQ SFRQHANLTQ HRRIHTGERP YACPECGKAF RQRPTLTQHL RTHRREKPFA CQDCGRRFHQ STKLIQHQRV HSAE
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分子量
82 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ZNF42, belonging to the zinc finger protein family, plays a critical role in various biological processes, including gene regulation, cell differentiation, and embryonic development. Its unique structure, characterized by multiple zinc finger motifs, enables it to interact with DNA and regulate target gene expression. Recent studies have highlighted ZNF42's involvement in pluripotency and self-renewal of stem cells, making it a significant focus in regenerative medicine and developmental biology. Understanding the mechanisms by which ZNF42 modulates gene activity can reveal insights into cellular reprogramming and potential therapeutic applications. Additionally, research on ZNF42 has implications for cancer biology, where its dysregulation may contribute to tumor progression. The recombinant protein production of ZNF42 is essential for characterizing its structure-function relationships and elucidating its biological roles. By expressing ZNF42 as a recombinant protein, researchers can perform detailed biochemical assays to investigate its DNA-binding properties, protein interactions, and effects on transcriptional regulation. Furthermore, the availability of purified ZNF42 facilitates high-throughput screening for small molecules that may modulate its activity, paving the way for novel therapeutic strategies targeting ZNF42-associated pathways. Overall, the study of ZNF42 recombinant proteins is crucial for advancing our understanding of this multifaceted transcription factor and its potential applications in biomedicine.
