Analytical Data
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基因名
TRIM45
- Application
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别名
FLJ13181; RING finger Protein 99; RNF99; TRI45_HUMAN; TRIM45; Tripartite motif containing 45; Tripartite motif containing Protein 45; Tripartite motif-containing Protein 45
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H8W5
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表达区间
1-580 aa
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氨基酸序列
MSENRKPLLG FVSKLTSGTA LGNSGKTHCP LCLGLFKAPR LLPCLHTVCT TCLEQLEPFS VVDIRGGDSD TSSEGSIFQE LKPRSLQSQI GILCPVCDAQ VDLPMGGVKA LTIDHLAVND VMLESLRGEG QGLVCDLCND REVEKRCQTC KANLCHFCCQ AHRRQKKTTY HTMVDLKDLK GYSRIGKPIL CPVHPAEELR LFCEFCDRPV CQDCVVGEHR EHPCDFTSNV IHKHGDSVWE LLKGTQPHVE ALEEALAQIH IINSALQKRV EAVAADVRTF SEGYIKAIEE HRDKLLKQLE DIRAQKENSL QLQKAQLEQL LADMRTGVEF TEHLLTSGSD LEILITKRVV VERLRKLNKV QYSTRPGVND KIRFCPQEKA GQCRGYEIYG TINTKEVDPA KCVLQGEDLH RAREKQTASF TLLCKDAAGE IMGRGGDNVQ VAVVPKDKKD SPVRTMVQDN KDGTYYISYT PKEPGVYTVW VCIKEQHVQG SPFTVMVRRK HRPHSGVFHC CTFCSSGGQK TARCACGGTM PGGYLGCGHG HKGHPGHPHW SCCGKFNEKS ECTWTGGQSA PRSLLRTVAL
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分子量
64.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TRIM45, a member of the tripartite motif (TRIM) protein family, has garnered attention in recent years due to its potential roles in various biological processes, including immune response, cell differentiation, and tumorigenesis. The TRIM proteins are characterized by their RING finger, B-box, and coiled-coil domains, which contribute to their E3 ubiquitin ligase activity, enabling them to regulate protein stability and signaling pathways through ubiquitination. Research indicates that TRIM45 might play a crucial role in modulating inflammatory responses and regulating the cell cycle, suggesting its involvement in both innate and adaptive immunity. Moreover, abnormal expression of TRIM45 has been linked to several cancers, raising questions about its function as a tumor suppressor or oncogene. Understanding the molecular mechanisms underlying TRIM45’s actions could illuminate its potential as a therapeutic target in diseases marked by dysregulated immunity and cancer. Consequently, the study of TRIM45 recombinant protein is essential for unraveling its biological functions and interactions, paving the way for novel interventions in medical research and therapeutic development.
