Analytical Data
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Gene name
PSMD4
- Application
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Alternative Names
PSMD4;MCB1;26S proteasome non-ATPase regulatory subunit 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P55036
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Expression Region
1-377aa
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AA Sequence
MVLESTMVCV DNSEYMRNGD FLPTRLQAQQ DAVNIVCHSK TRSNPENNVG LITLANDCEV LTTLTPDTGR ILSKLHTVQP KGKITFCTGI RVAHLALKHR QGKNHKMRII AFVGSPVEDN EKDLVKLAKR LKKEKVNVDI INFGEEEVNT EKLTAFVNTL NGKDGTGSHL VTVPPGPSLA DALISSPILA GEGGAMLGLG ASDFEFGVDP SADPELALAL RVSMEEQRQR QEEEARRAAA ASAAEAGIAT TGTEDSDDAL LKMTISQQEF GRTGLPDLSS MTEEEQIAYA MQMSLQGAEF GQAESADIDA SSAMDTSEPA KEEDDYDVMQ DPEFLQSVLE NLPGVDPNNE AIRNAMGSLA SQATKDGKKD KKEEDKK
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PSMD4, a member of the 19S regulatory complex of the proteasome, plays a critical role in protein degradation and cellular homeostasis. Its involvement in various cellular processes, such as cell cycle regulation, stress responses, and signal transduction, elevates its significance in maintaining proteostasis. Recent studies have indicated that dysregulation of PSMD4 is linked to several diseases, including cancer and neurodegenerative disorders, making it a potential biomarker and therapeutic target. Understanding the structure and function of PSMD4 through recombinant protein studies can provide insights into its mechanistic role in proteasomal activity and its interaction with other cellular components. By examining the biochemical properties and functional implications of PSMD4, researchers aim to reveal novel pathways that could lead to targeted treatments. Moreover, the use of PSMD4 recombinant protein allows for the exploration of its role in protein-protein interactions and post-translational modifications, thus enhancing our understanding of its contribution to cellular integrity and disease manifestation. This research not only has implications for basic biological understanding but also holds promise for the development of innovative therapeutic strategies for diseases associated with proteasomal dysfunction.











