Analytical Data
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基因名
ACTN4
- Application
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别名
ACTN4；Alpha-actinin-4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43707
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表达区间
397-648aa
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氨基酸序列
EEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNE
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分子量
29 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACTN4 (α-actinin-4) is a member of the actin-binding protein family, primarily involved in the organization of the cytoskeleton and the regulation of cellular processes such as migration, adhesion, and signal transduction. It is encoded by the ACTN4 gene and is expressed in various tissues, playing a critical role in maintaining cell structure and facilitating cellular responses to mechanical stress. Research has highlighted its importance in various pathological conditions, including cancer metastasis, where ACTN4 is often overexpressed, promoting tumor cell invasion and migration. The protein has also been implicated in kidney diseases and cardiovascular disorders. The recombinant production of ACTN4 has allowed for detailed studies of its structural and functional properties, enabling researchers to explore its role in cellular dynamics and disease mechanisms. Understanding the biophysical properties of ACTN4, including its interaction with actin and other cellular components, is essential for designing potential therapeutic strategies targeting its pathways. As a result, the study of recombinant ACTN4 protein serves as a critical tool in elucidating its biological functions and contributions to disease, paving the way for the development of innovative intervention strategies.
