Analytical Data
-
Gene name
PARP4
- Application
-
Alternative Names
PARP4；ADPRTL1；KIAA0177；PARPL；Protein mono-ADP-ribosyltransferase PARP4
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9UKK3
-
Expression Region
607-1046aa
-
AA Sequence
SSTKAGLQDASGNLVPLEDVHIKGRIIDTVAQVIVFQTYTNKSHVPIEAKYIFPLDDKAAVCGFEAFINGKHIVGEIKEKEEAQQEYLEAVTQGHGAYLMSQDAPDVFTVSVGNLPPKAKVLIKITYITELSILGTVGVFFMPATVAPWQQDKALNENLQDTVEKICIKEIGTKQSFSLTMSIEMPYVIEFIFSDTHELKQKRTDCKAVISTMEGSSLDSSGFSLHIGLSAAYLPRMWVEKHPEKESEACMLVFQPDLDVDLPDLASESEVIICLDCSSSMEGVTFLQAKQIALHALSLVGEKQKVNIIQFGTGYKELFSYPKHITSNTMAAEFIMSATPTMGNTDFWKTLRYLSLLYPARGSRNILLVSDGHLQDESLTLQLVKRSRPHTRLFACGIGSTANRHVLRILSQCGAGVFEYFNAKSKHSWRKQIEDQMTRL
-
Molecular Weight
55.0 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PARP4 (Poly(ADP-ribose) polymerase 4) is a member of the PARP protein family, known for its role in cellular processes such as DNA repair, apoptosis, and gene regulation. Unlike its better-studied relatives, PARP1 and PARP2, which are primarily associated with the repair of DNA damage, PARP4 has emerged as a protein of interest due to its unique structural features and diverse functions. Recent research indicates that PARP4 plays a critical role in the regulation of cell survival, proliferation, and development, particularly in response to environmental stressors. It is speculated that PARP4 may be involved in modulating the cell's response to DNA damage and maintaining genomic stability, thus influencing tumorigenesis. Furthermore, studies have suggested that PARP4 interacts with various proteins, including transcription factors and chromatin remodelers, implicating it in the regulation of gene expression. These insights have prompted researchers to investigate the potential of PARP4 as a therapeutic target in cancer treatment, especially in tumors characterized by dysregulated DNA repair mechanisms. As a result, understanding the functional roles and mechanisms of PARP4 through recombinant protein studies is crucial for elucidating its biological implications and therapeutic potentials.
