Analytical Data
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基因名
ESPL1
- Application
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别名
Caspase like protein ESPL1; Caspase-like protein ESPL1; ESP 1; ESP1; ESPL 1; Espl1; ESPL1_HUMAN; Extra spindle poles like 1; Extra spindle poles like 1 protein; Extra spindle poles-like 1 protein; FLJ46492; KIAA0165; Separase; Separin; Similar to fission yeast cut1and gene; SSE
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q14674
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表达区间
586-701aa
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氨基酸序列
REELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKIQEGIERDR
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分子量
38.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ESPL1, also known as separase, is a crucial enzyme involved in the regulation of cell division, specifically during the anaphase stage of mitosis. Its primary function is to cleave cohesin, the protein complex that holds sister chromatids together, thereby facilitating their separation and ensuring accurate chromosomal segregation. Dysregulation of ESPL1 can lead to various cellular dysfunctions and is implicated in oncogenesis; overexpression has been linked to several types of cancer, while mutations can cause chromosomal instability. Given its significance in cell cycle regulation and tumor development, research on ESPL1 recombinant proteins has intensified. These studies aim to elucidate the detailed mechanisms governing ESPL1 activity, its interactions with other key proteins, and its role in the pathophysiology of cancer. Recombinant ESPL1 proteins are employed in structural and functional assays that provide insights into the enzymatic activity and regulatory pathways associated with this enzyme. Furthermore, understanding ESPL1’s role in chromosomal dynamics may reveal potential therapeutic targets for cancer treatment, making it a focal point in cancer biology and drug development. The exploration of ESPL1 also extends to investigating its potential role in other cellular processes beyond mitosis, positioning it as a versatile protein of interest in molecular biology and cancer research.
