Analytical Data
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Gene name
ESPL1
- Application
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Alternative Names
Caspase like protein ESPL1; Caspase-like protein ESPL1; ESP 1; ESP1; ESPL 1; Espl1; ESPL1_HUMAN; Extra spindle poles like 1; Extra spindle poles like 1 protein; Extra spindle poles-like 1 protein; FLJ46492; KIAA0165; Separase; Separin; Similar to fission yeast cut1and gene; SSE
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14674
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Expression Region
586-701aa
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AA Sequence
REELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKIQEGIERDR
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Molecular Weight
38.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ESPL1, also known as separase, is a crucial enzyme involved in the regulation of cell division, specifically during the anaphase stage of mitosis. Its primary function is to cleave cohesin, the protein complex that holds sister chromatids together, thereby facilitating their separation and ensuring accurate chromosomal segregation. Dysregulation of ESPL1 can lead to various cellular dysfunctions and is implicated in oncogenesis; overexpression has been linked to several types of cancer, while mutations can cause chromosomal instability. Given its significance in cell cycle regulation and tumor development, research on ESPL1 recombinant proteins has intensified. These studies aim to elucidate the detailed mechanisms governing ESPL1 activity, its interactions with other key proteins, and its role in the pathophysiology of cancer. Recombinant ESPL1 proteins are employed in structural and functional assays that provide insights into the enzymatic activity and regulatory pathways associated with this enzyme. Furthermore, understanding ESPL1’s role in chromosomal dynamics may reveal potential therapeutic targets for cancer treatment, making it a focal point in cancer biology and drug development. The exploration of ESPL1 also extends to investigating its potential role in other cellular processes beyond mitosis, positioning it as a versatile protein of interest in molecular biology and cancer research.
