Analytical Data
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Gene name
AFMID
- Application
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Alternative Names
AFMID；Kynurenine formamidase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q63HM1
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Expression Region
1-303aa
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AA Sequence
MMDVSGVGFP SKVPWKKMSA EELENQYCPS RWVVRLGAEE ALRTYSQIGI EATTRARATR KSLLHVPYGD GEGEKVDIYF PDESSEALPF FLFFHGGYWQ SGSKDESAFM VHPLTAQGVA VVIVAYGIAP KGTLDHMVDQ VTRSVAFVQK RYPSNKGIYL CGHSAGAHLA AMMLLADWTK HGVTPNLRGF FLVSGVFDLE PIVYTSQNVA LQLTLEDAQR NSPQLKVAQA QPVDPTCRVL VVVGQFDSPE FHRQSWEFYQ TLCQGEWKAS FEELHDVDHF EIVENLTQKD NVLTQIILKT IFQ
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AFMID, or arylformamidase, is an enzyme that plays a crucial role in the metabolism of endogenous and exogenous substances, particularly in the biodegradation of arylamide compounds. Research on AFMID has gained significance due to its involvement in various physiological processes, including the regulation of neurotransmitter levels and the potential detoxification pathways for harmful substances. Studies suggest that AFMID activity may influence neurological health and disease, where aberrant enzyme functioning is linked to conditions like Alzheimer's disease and other neurodegenerative disorders. Furthermore, interest in AFMID’s role in drug metabolism has increased, as it may serve as a biomarker for individual responses to specific pharmaceuticals, influencing drug efficacy and safety profiles. To explore these aspects, scientists have focused on the recombinant production of AFMID proteins, allowing for detailed biochemical characterization, enzyme kinetics studies, and structure-function analyses. By utilizing advanced genetic engineering techniques, researchers aim to produce higher yields of functional AFMID, facilitating the exploration of its enzymatic mechanisms and regulatory roles in metabolic pathways. This research not only enhances our understanding of AFMID's biological significance but also opens avenues for therapeutic interventions targeting related metabolic disorders and improving drug development strategies.
