Analytical Data
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基因名
TMEM175
- Application
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别名
TMEM175; Endosomal/lysosomal potassium channel TMEM175; Transmembrane Protein 175; hTMEM175
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BSA9
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表达区间
1-504 aa
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氨基酸序列
MSQPRTPEQALDTPGDCPPGRRDEDAGEGIQCSQRMLSFSDALLSIIATVMILPVTHTEISPEQQFDRSVQRLLATRIAVYLMTFLIVTVAWAAHTRLFQVVGKTDDTLALLNLACMMTITFLPYTFSLMVTFPDVPLGIFLFCVCVIAIGVVQALIVGYAFHFPHLLSPQIQRSAHRALYRRHVLGIVLQGPALCFAAAIFSLFFVPLSYLLMVTVILLPYVSKVTGWCRDRLLGHREPSAHPVEVFSFDLHEPLSKERVEAFSDGVYAIVATLLILDICEDNVPDPKDVKERFSGSLVAALSATGPRFLAYFGSFATVGLLWFAHHSLFLHVRKATRAMGLLNTLSLAFVGGLPLAYQQTSAFARQPRDELERVRVSCTIIFLASIFQLAMWTTALLHQAETLQPSVWFGGREHVLMFAKLALYPCASLLAFASTCLLSRFSVGIFHLMQIAVPCAFLLLRLLVGLALATLRVLRGLARPEHPPPAPTGQDDPQSQLLPAPC
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分子量
82 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TMEM175 is a member of the transmembrane protein family, predominantly expressed in neurons, and is believed to play a crucial role in maintaining ion homeostasis and cellular pH. Recent studies have linked TMEM175 to various neurodegenerative diseases, including Parkinson's disease, suggesting that its dysfunction might contribute to the pathogenesis of these conditions. Researchers have identified TMEM175 as a lysosomal ion channel, essential for regulating the transport of ions such as potassium and chloride across cellular membranes. The reconstitution of TMEM175 protein in vitro is essential for understanding its structure-function relationships and its physiological roles. Efforts to express and purify recombinant TMEM175 protein have been initiated to investigate its ion channel activity, gating mechanisms, and interactions with other cellular components. Such studies are crucial, as they may unveil novel therapeutic targets for treating neurodegenerative disorders associated with TMEM175 mutations or dysregulation. Furthermore, utilizing advanced techniques like electrophysiology and structural biology will enhance our understanding of this protein's contribution to neuronal health and highlight the importance of ion channels in cellular homeostasis, thereby opening new avenues for the development of innovative treatment strategies.
