Analytical Data
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Gene name
PKCi
- Application
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Alternative Names
PKCi；Adenosine 5'-monophosphoramidase HINT2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P41743
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Expression Region
2-596aa
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AA Sequence
PTQRDSSTM SHTVAGGGSG DHSHQVRVKA YYRGDIMITH FEPSISFEGL CNEVRDMCSF DNEQLFTMKW IDEEGDPCTV SSQLELEEAF RLYELNKDSE LLIHVFPCVP ERPGMPCPGE DKSIYRRGAR RWRKLYCANG HTFQAKRFNR RAHCAICTDR IWGLGRQGYK CINCKLLVHK KCHKLVTIEC GRHSLPQEPV MPMDQSSMHS DHAQTVIPYN PSSHESLDQV GEEKEAMNTR ESGKASSSLG LQDFDLLRVI GRGSYAKVLL VRLKKTDRIY AMKVVKKELV NDDEDIDWVQ TEKHVFEQAS NHPFLVGLHS CFQTESRLFF VIEYVNGGDL MFHMQRQRKL PEEHARFYSA EISLALNYLH ERGIIYRDLK LDNVLLDSEG HIKLTDYGMC KEGLRPGDTT STFCGTPNYI APEILRGEDY GFSVDWWALG VLMFEMMAGR SPFDIVGSSD NPDQNTEDYL FQVILEKQIR IPRSLSVKAA SVLKSFLNKD PKERLGCHPQ TGFADIQGHP FFRNVDWDMM EQKQVVPPFK PNISGEFGLD NFDSQFTNEP VQLTPDDDDI VRKIDQSEFE GFEYINPLLM SAEECV
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PKCi, or Protein Kinase C iota, is a member of the Protein Kinase C (PKC) family, which plays a critical role in various cellular processes, including growth, differentiation, and apoptosis. Research on PKCi has gained traction due to its involvement in several pathological conditions, particularly in cancer progression and inflammation. Unlike other PKC isoforms, PKCi is predominantly expressed in certain tissues, such as the brain and immune cells, making it a potential therapeutic target for diseases where these tissues are affected. Its unique signaling pathways have been shown to influence tumor cell survival and proliferation, suggesting that dysregulation of PKCi activity might contribute to oncogenesis. Additionally, recent studies have indicated that PKCi may be involved in mediating resistance to chemotherapy, providing further impetus to explore its role in cancer therapy. The development of recombinant PKCi proteins has become essential for understanding its molecular mechanisms and interactions, enabling researchers to devise targeted strategies for drug development. Thus, the study of PKCi recombinant proteins not only enhances our knowledge of its biological functions but also opens new avenues for therapeutic interventions in various diseases, highlighting its significance in biomedical research.
