Analytical Data
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Gene name
API5
- Application
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Alternative Names
API5；Apoptosis inhibitor 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BZZ5
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Expression Region
2-524aa
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AA Sequence
PTVEELYRN YGILADATEQ VGQHKDAYQV ILDGVKGGTK EKRLAAQFIP KFFKHFPELA DSAINAQLDL CEDEDVSIRR QAIKELPQFA TGENLPRVAD ILTQLLQTDD SAEFNLVNNA LLSIFKMDAK GTLGGLFSQI LQGEDIVRER AIKFLSTKLK TLPDEVLTKE VEELILTESK KVLEDVTGEE FVLFMKILSG LKSLQTVSGR QQLVELVAEQ ADLEQTFNPS DPDCVDRLLQ CTRQAVPLFS KNVHSTRFVT YFCEQVLPNL GTLTTPVEGL DIQLEVLKLL AEMSSFCGDM EKLETNLRKL FDKLLEYMPL PPEEAENGEN AGNEEPKLQF SYVECLLYSF HQLGRKLPDF LTAKLNAEKL KDFKIRLQYF ARGLQVYIRQ LRLALQGKTG EALKTEENKI KVVALKITNN INVLIKDLFH IPPSYKSTVT LSWKPVQKVE IGQKRASEDT TSGSPPKKSS AGPKRDARQI YNPPSGKYSS NLGNFNYEQR GAFRGSRGGR GWGTRGNRSR GRLY
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
API5 (Apoptosis Inhibitor 5) is a member of the anti-apoptotic Bcl-2 protein family, known for its role in promoting cell survival by inhibiting apoptosis, a process essential for maintaining cellular homeostasis and tissue integrity. Research has identified API5 as a key player in various cancers, where its overexpression is often linked to tumor progression, resistance to chemotherapy, and poor patient prognosis. The protein's mechanism involves the modulation of several signaling pathways, including those related to survival, proliferation, and differentiation. Given its significant implications in cancer biology, API5 has emerged as a potential therapeutic target. Efforts to engineer recombinant API5 proteins aim to elucidate their structure-function relationship, understand their interactions with other cellular proteins, and develop API5-based therapeutics. These studies include the optimization of expression systems for recombinant protein production, followed by purification and characterization using techniques such as X-ray crystallography and NMR spectroscopy. Additionally, investigations into API5 inhibitors or applications of CRISPR-Cas9 for gene editing provide further avenues for exploring its functional role in cancer therapy. Overall, the comprehensive study of API5 through recombinant protein technology promises to advance our understanding of its biological functions and therapeutic potential in malignancies.
