Analytical Data
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Gene name
EIF4G3
- Application
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Alternative Names
eIF-4-gamma 3; eIF-4-gamma II; eIF-4G 3; eIF4G 3; EIF4G3; eIF4GII; Eukaryotic translation initiation factor 4 gamma 3; eukaryotic translation initiation factor 4 gamma; 3; eukaryotic translation initiation factor-4 gamma; 3; IF4G3_HUMAN
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43432
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Expression Region
1-515aa
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AA Sequence
MNSQPQTRSPFAAGPRPPHHQFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYPVPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIMVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIISGGGSRNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDGDICKKPCSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLNSRRSPVPETSNEC
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Molecular Weight
82.39 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EIF4G3, or eukaryotic translation initiation factor 4 gamma 3, is a critical component of the eukaryotic translation initiation complex, playing a pivotal role in protein synthesis by mediating the interaction between mRNA and ribosomes. Recent studies have highlighted its significance in various cellular processes, including cell proliferation and apoptosis. The recombinant production of EIF4G3 has garnered attention for its potential applications in understanding the mechanisms of translation initiation and its regulation under pathological conditions. Given the involvement of EIF4G3 in cancer and viral infections, researchers aim to dissect its functional domains and interaction partners through recombinant protein studies. These investigations could reveal insights into its role in oncogenesis and viral replication. Furthermore, the production of EIF4G3 in a recombinant form allows for advanced biochemical assays, providing a platform for drug discovery and therapeutic interventions targeting translation machinery. Understanding the structure and function of EIF4G3 not only elucidates fundamental aspects of translation regulation but also opens avenues for developing novel strategies in cancer therapy and antiviral drug design. Thus, ongoing research efforts focus on optimizing the expression and purification of recombinant EIF4G3 to facilitate comprehensive functional analyses and its potential exploitation in biomedical applications.
