Analytical Data
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Gene name
EIF2C2
- Application
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Alternative Names
Ago 2; AGO2_HUMAN; Argonaute 2; argonaute 2; RISC catalytic component; Argonaute RISC catalytic component 2; Argonaute2; CTA-204B4.6; dAgo2; eIF 2C 2; eIF-2C 2; eIF2C 2; Eif2c2; Eukaryotic translation initiation factor 2C 2; Eukaryotic translation initiation factor 2C subunit 2
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UKV8
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Expression Region
517-818aa
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AA Sequence
LVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLV
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Molecular Weight
36.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF2C2, also known as Argonaute 2 (Ago2), is a key player in the RNA interference (RNAi) pathway, which regulates gene expression and maintains cellular homeostasis. As a component of the RNA-induced silencing complex (RISC), EIF2C2 is involved in the incorporation of small, non-coding RNAs, particularly microRNAs (miRNAs) and small interfering RNAs (siRNAs), which guide the RISC to target messenger RNAs (mRNAs) for cleavage or translational repression. The proper function of EIF2C2 is crucial for various biological processes, including development, differentiation, and the response to stress. Misregulation of this protein has been implicated in numerous diseases, including cancer, neurological disorders, and viral infections. Therefore, research on the recombinant expression and functional analysis of EIF2C2 is essential for understanding its role in RNA silencing mechanisms and its potential as a therapeutic target. Recombinant EIF2C2 protein can be used to elucidate the molecular mechanisms of RNAi, assess its interactions with various miRNAs and mRNAs, and explore possible pharmacological interventions that modulate its activity. Advances in this area of study could lead to innovative strategies for gene regulation and the development of novel RNA-based therapeutics.
