Cat: PA1000-8174

Recombinant Human TSHR Protein,His

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Analytical Data

  • Gene name

    TSHR

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    TSHR;LGR3;Thyrotropin receptor

  • Species

    Human

  • Source

    E. coli

  • Tag

    N-terminal His-Tag

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P16473

  • Expression Region

    21-413aa

  • AA Sequence

    GMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSN LPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLL KFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTL KLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQT SVTALPSKGLEHLKELIARNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQK KIRGILESLMCNESSMQSLRQRKSVNALNSPLHQEYEENLGDSIVGYKEKSKFQ DTHNNAHYYVFFEEQEDEIIGFGQELKNPQEETLQAFDSHYDYTICGDSEDMVC TPKSDEFNPCEDIMG

  • Molecular Weight

    48.3kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

Thyroid-stimulating hormone receptor (TSHR) plays a crucial role in regulating thyroid function and maintaining metabolic homeostasis by mediating the effects of thyroid-stimulating hormone (TSH). Dysregulation of TSHR signaling is implicated in various thyroid disorders, including hyperthyroidism and hypothyroidism, as well as autoimmune conditions like Graves' disease and Hashimoto's thyroiditis. Given its significance, researchers have been keen on studying TSHR to unravel its complex signaling mechanisms and structural characteristics. The generation of recombinant TSHR proteins has become a key focus, as these proteins serve as essential tools for investigating receptor behavior, drug interactions, and antibody development. Utilizing recombinant DNA technology, scientists can produce active TSHR proteins that mimic the natural receptor, facilitating biochemical assays and structural biology studies. The insights gained from TSHR recombinant studies can lead to novel therapeutic strategies and improve our understanding of thyroid pathophysiology, ultimately contributing to better management of thyroid-related diseases and enhancing patient outcomes.

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