Cat: PA2000-7296

Recombinant Human EHD3 Protein,GST

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Analytical Data

  • Gene name

    EHD3

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    EHD3; EHD2; PAST3EH domain-containing protein 3; PAST homolog 3

  • Species

    Human

  • Source

    E. coli

  • Tag

    GST-tag at N-terminal

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9NZN3

  • Expression Region

    1-535aa

  • AA Sequence

    MFSWLGTDDR RRKDPEVFQT VSEGLKKLYK SKLLPLEEHY RFHEFHSPAL EDADFDNKPM VLLVGQYSTG KTTFIRYLLE QDFPGMRIGP EPTTDSFIAV MQGDMEGIIP GNALVVDPKK PFRKLNAFGN AFLNRFVCAQ LPNPVLESIS VIDTPGILSG EKQRISRGYD FAAVLEWFAE RVDRIILLFD AHKLDISDEF SEVIKALKNH EDKMRVVLNK ADQIETQQLM RVYGALMWSL GKIVNTPEVI RVYIGSFWSH PLLIPDNRKL FEAEEQDLFR DIQSLPRNAA LRKLNDLIKR ARLAKVHAYI ISSLKKEMPS VFGKDNKKKE LVNNLAEIYG RIEREHQISP GDFPNLKRMQ DQLQAQDFSK FQPLKSKLLE VVDDMLAHDI AQLMVLVRQE ESQRPIQMVK GGAFEGTLHG PFGHGYGEGA GEGIDDAEWV VARDKPMYDE IFYTLSPVDG KITGANAKKE MVRSKLPNSV LGKIWKLADI DKDGMLDDDE FALANHLIKV KLEGHELPNE LPAHLLPPSK RKVAE

  • Molecular Weight

    60.8 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

EHD3 (EH domain-containing protein 3) is a member of the EHD family of proteins known for their roles in endocytic and intracellular trafficking pathways. The significance of EHD3 in cellular processes has garnered attention in recent years, particularly due to its involvement in clathrin-mediated endocytosis and the regulation of endosomal transport. Research indicates that EHD3 plays a critical role in maintaining cellular homeostasis, influencing functions such as membrane remodeling, vesicle formation, and cargo sorting. Abnormalities in EHD3 expression or function have been linked to various diseases, including cancer and neurodegenerative disorders, making it a potential target for therapeutic interventions. The characterization of EHD3 recombinant proteins provides valuable insights into its structural and functional dynamics, elucidating the molecular mechanisms underlying its cellular roles. Understanding the biophysical properties and interaction networks of EHD3 can contribute to the development of novel strategies aimed at manipulating its activities for therapeutic purposes, emphasizing the importance of this protein in the study of cellular dynamics and disease mechanisms.

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