Analytical Data
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基因名
EDEM1
- Application
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别名
EDEM1; EDEM; KIAA0212ER degradation-enhancing alpha-mannosidase-like protein 1
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q92611
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表达区间
559-656
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氨基酸序列
YLLFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGL
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分子量
36.52 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EDEM1 (ER degradation-enhancing α-mannosidase I-like protein 1) is a crucial component of the endoplasmic reticulum (ER) associated degradation (ERAD) pathway, which is responsible for the quality control of glycoproteins. Proteins that are misfolded or improperly glycosylated are targeted for degradation to maintain cellular homeostasis. EDEM1 specifically functions in recognizing and extracting these defective glycoproteins from the ER lumen, facilitating their transport to the proteasome for degradation. The research surrounding EDEM1 has gained significance due to its potential implications in various diseases, including neurodegenerative disorders and cancer, where protein misfolding and aggregation are common. Furthermore, EDEM1’s role in the immune response and its influence on glycoprotein maturation highlight its importance in cellular functions. Studies have also explored the structural and functional mechanisms of EDEM1, helping to elucidate its interactions with glycoproteins and other components of the ERAD pathway. Understanding EDEM1 can provide insights into therapeutic strategies for diseases associated with protein misfolding, making it a significant focus of biomedical research.
