Analytical Data
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Gene name
ZBTB16
- Application
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Alternative Names
Zinc finger and BTB domain-containing Protein 16. Promyelocytic leukemia zinc finger Protein. Zinc finger Protein 145. Zinc finger Protein PLZF
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q05516
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Expression Region
1-673 aa
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AA Sequence
MDLTKMGMIQLQNPSHPTGLLCKANQMRLAGTLCDVVIMVDSQEFHAHRTVLACTSKMFEILFHRNSQHYTLDFLSPKTFQQILEYAYTATLQAKAEDLDDLLYAAEILEIEYLEEQCLKMLETIQASDDNDTEATMADGGAEEEEDRKARYLKNIFISKHSSEESGYASVAGQSLPGPMVDQSPSVSTSFGLSAMSPTKAAVDSLMTIGQSLLQGTLQPPAGPEEPTLAGGGRHPGVAEVKTEMMQVDEVPSQDSPGAAESSISGGMGDKVEERGKEGPGTPTRSSVITSARELHYGREESAEQVPPPAEAGQAPTGRPEHPAPPPEKHLGIYSVLPNHKADAVLSMPSSVTSGLHVQPALAVSMDFSTYGGLLPQGFIQRELFSKLGELAVGMKSESRTIGEQCSVCGVELPDNEAVEQHRKLHSGMKTYGCELCGKRFLDSLRLRMHLLAHSAGAKAFVCDQCGAQFSKEDALETHRQTHTGTDMAVFCLLCGKRFQAQSALQQHMEVHAGVRSYICSECNRTFPSHTALKRHLRSHTGDHPYECEFCGSCFRDESTLKSHKRIHTGEKPYECNGCGKKFSLKHQLETHYRVHTGEKPFECKLCHQRSRDYSAMIKHLRTHNGASPYQCTICTEYCPSLSSMQKHMKGHKPEEIPPDWRIEKTYLYLCYV
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Molecular Weight
74.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ZBTB16, also known as promyelocytic leukemia zinc finger protein (PLZF), is a member of the BTB/POZ (Broad-Complex, Tramtrack, and Bric-a-brac/Poxvirus and Zinc finger) protein family, which plays critical roles in gene regulation and cellular differentiation. Its primary expression is observed in immune cells, particularly in T cells and natural killer (NK) cells, where it is involved in regulating developmental processes and adaptive immune responses. Abnormalities in ZBTB16 expression have been associated with various hematological malignancies, notably acute promyelocytic leukemia (APL), where fusion proteins resulting from chromosomal translocations disrupt normal gene function and contribute to oncogenesis. Given its pivotal role in hematopoiesis and immune function, research on recombinant ZBTB16 proteins aims to elucidate its mechanisms of action and potential therapeutic applications. Understanding ZBTB16's structure and function through recombinant protein studies can shed light on its role in cellular signaling pathways, transcriptional regulation, and epigenetic modifications. Moreover, recombinant ZBTB16 proteins can serve as valuable tools for developing targeted therapies and biomarkers for diagnosing and treating diseases associated with ZBTB16 dysregulation, particularly in the context of leukemia and other cancers. As such, ongoing studies focus on the functional characterization of ZBTB16 and its interactions with other proteins, with the goal of translating these insights into clinical applications.
